Description

Z-Ala-Ala-Lys-Oh is a synthetic tripeptide derivative featuring a benzyloxycarbonyl (Z) protecting group on the N-terminus and a free carboxylic acid on the C-terminus. This compound serves as a crucial building block and biochemical tool in advanced research and development, particularly for studying protease specificity and enzyme kinetics. It is primarily utilized by pharmaceutical researchers, peptide chemists, and diagnostic developers working on enzyme inhibitors, substrate libraries, and novel therapeutic agents.

Application

• Biochemical Research: As a specific chromogenic or fluorogenic substrate for studying the activity and inhibition profiles of serine proteases, including trypsin and trypsin-like enzymes.
• Pharmaceutical Development: A key intermediate in the synthesis of more complex peptide-based drug candidates and protease inhibitor libraries.
• Diagnostic Reagent Manufacturing: Used in the formulation of enzyme assay kits for clinical diagnostics and life science research to measure protease activity.
• Enzyme Characterization: Employed in academic and industrial laboratories to determine kinetic parameters (Km, kcat) and elucidate enzyme mechanisms.
• Peptide Chemistry: Acts as a protected fragment for solid-phase or solution-phase peptide synthesis (SPPS) to construct larger peptide sequences.

Basic Information

Product Name	Z-Ala-Ala-Lys-Oh
CAS No.	252336-23-5
Molecular Formula	C₂₂H₃₄N₄O₆
Molecular Weight	450.53 g/mol
Synonyms	Nα-Benzyloxycarbonyl-L-alanyl-L-alanyl-L-lysine; Z-Ala-Ala-Lys-OH; Z-A-A-K-OH; Cbz-Ala-Ala-Lys-OH; Benzyloxycarbonyl-Ala-Ala-Lys; N-Cbz-L-alanyl-L-alanyl-L-lysine; L-Lysine, N2-[(benzyloxy)carbonyl]-L-alanyl-L-alanyl-; Substrate for trypsin-like proteases
EINECS	Contact for details

Quality Control

Every batch of Z-Ala-Ala-Lys-Oh is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our quality commitment includes providing comprehensive Certificates of Analysis (COA) with each shipment, detailing results from HPLC, NMR, and MS. We adhere to cGMP principles where applicable to support our clients in research and development phases.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.