Description

Fmoc-D-Cys(Mtt)-Oh CAS NO 252206-29-4 is a protected, enantiomerically pure D-cysteine derivative specifically engineered for solid-phase peptide synthesis (SPPS). This compound is a critical building block for the controlled introduction of cysteine residues, enabling the synthesis of complex peptides and proteins with defined disulfide bridges. It is essential for researchers and manufacturers in pharmaceutical development, biotechnology, and advanced materials science who require high-purity, sequence-specific amino acids for constructing bioactive molecules.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key protected amino acid for the Fmoc/t-Bu strategy, allowing for the sequential assembly of peptide chains on resin.
• Pharmaceutical Intermediate: Used in the research and production of therapeutic peptides, including hormone analogs, antimicrobial peptides, and targeted drug conjugates.
• Disulfide Bridge Formation: The orthogonal protecting groups (Fmoc for α-amine, Mtt for thiol) enable precise, post-assembly deprotection and oxidation to form critical disulfide bonds in peptides like oxytocin, insulin analogs, and conotoxins.
• Bioconjugation and Labeling: The free thiol, after deprotection, serves as a specific handle for site-directed conjugation with maleimides, iodoacetamides, or other agents for creating fluorescent probes, PEGylated drugs, or antibody-drug conjugates (ADCs).
• Chemical Biology Research: Employed in studying protein-protein interactions, enzyme mechanisms, and structure-activity relationships (SAR) by incorporating non-natural or modified cysteine residues.
• Material Science: Used to synthesize peptide-based biomaterials, hydrogels, and surface coatings where cysteine residues provide cross-linking points or adhesion sites.

Basic Information

Product Name	Fmoc-D-Cys(Mtt)-Oh
CAS No.	252206-29-4
Molecular Formula	C35H33NO5S
Molecular Weight	579.70 g/mol
Synonyms	Fmoc-D-cysteine(Mtt)-OH; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-3-[(4-methylphenyl)diphenylmethyl]thio-D-alanine; Fmoc-D-Cys(4-Methyltrityl)-OH; Fmoc-D-Cysteine(Mtt)-OH; Nα-Fmoc-S-(4-methyltrityl)-D-cysteine; Fmoc-D-Cys(Mmt)-OH; (R)-2-(((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-3-((4-methylphenyl)diphenylmethyl)thio)propanoic acid
EINECS	Contact for details

Quality Control

Our Fmoc-D-Cys(Mtt)-Oh is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and chiral purity, NMR for structural confirmation, and MS for identity verification. We provide Certificates of Analysis (COA) detailing all specifications to ensure compliance with research and cGMP guidelines for advanced intermediates. Our quality commitment ensures batch-to-batch consistency and reliability for your critical synthesis workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to minimize moisture condensation. For long-term stability, maintain the recommended cold chain.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Chiral Purity (HPLC)	≥ 99.0% (D-isomer)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.