Description

Fmoc-His(3-Me)-Oh CAS NO 252049-16-4 is a protected derivative of the amino acid histidine, specifically modified at the 3-position of the imidazole ring with a methyl group and featuring an Fmoc (9-fluorenylmethoxycarbonyl) protecting group on the α-amine. This compound is critically important for solid-phase peptide synthesis (SPPS), where it enables the controlled and sequential assembly of peptide chains containing modified histidine residues. It is an essential building block for researchers and manufacturers in pharmaceutical development, biochemical research, and the production of therapeutic peptides and peptidomimetics.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key protected amino acid for the automated synthesis of complex peptides, ensuring the integrity of the histidine side chain during coupling and deprotection cycles.
• Pharmaceutical Research & Development: Used in the discovery and production of peptide-based drug candidates, including hormones, enzyme inhibitors, and targeted therapeutics.
• Bioconjugation and Protein Engineering: Serves as a precursor for introducing site-specific modifications into proteins and peptides to study function or alter pharmacokinetic properties.
• Combinatorial Chemistry Libraries: Employed to generate diverse arrays of peptide compounds for high-throughput screening against biological targets.
• Academic & Biochemical Research: Essential for fundamental studies in peptide chemistry, structure-activity relationships (SAR), and molecular biology.
• Production of Diagnostic Peptides: Used in the synthesis of peptide antigens and other components for diagnostic assays and kits.

Basic Information

Item	Detail
Product Name	Fmoc-His(3-Me)-Oh
CAS No.	252049-16-4
Molecular Formula	C22H22N4O4
Molecular Weight	406.44 g/mol
Synonyms	Fmoc-3-methyl-L-histidine; N-α-Fmoc-3-methyl-L-histidine; (S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-3-(1-methyl-1H-imidazol-5-yl)propanoic acid; Fmoc-His(3-Me)-OH; Fmoc-3-Me-His-OH; N-Fmoc-3-methylhistidine; Fmoc-L-His(3-Me)-OH; Fmoc-protected 3-methylhistidine
EINECS	Contact for details

Quality Control

Our Fmoc-His(3-Me)-Oh is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistency, meeting the exacting standards required for peptide synthesis. Certificates of Analysis (COA) providing detailed results for purity, chiral integrity, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Rotation	-10.0° to -14.0° (c=1 in DMF)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.