Description

Z-Val-Ala-Asn-Amc CAS NO 245036-58-2 is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly for enzymes like legumain or other asparaginyl endopeptidases. It is an essential tool for biochemical research, pharmaceutical development, and diagnostic applications where accurate quantification of enzymatic kinetics is paramount.

Application

• Protease Activity Assays: Primary substrate for fluorometrically monitoring the activity of legumain and related cysteine proteases.
• Biochemical Research: Used in enzyme characterization, inhibitor screening, and mechanistic studies of proteolytic pathways.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) campaigns to identify and validate novel therapeutic protease inhibitors.
• Diagnostic Reagent Development: Serves as a key component in developing sensitive in-vitro diagnostic tests for diseases associated with dysregulated protease activity.
• Cell Biology Studies: Used to detect and quantify specific protease activity within live cells or cell lysates.
• Quality Control: Acts as a reference standard in the QC of recombinant protease production and related biopharmaceuticals.

Basic Information

Product Name	Z-Val-Ala-Asn-Amc
CAS No.	245036-58-2
Molecular Formula	C26H36N6O8
Molecular Weight	560.60 g/mol
Synonyms	Z-Val-Ala-Asn-AMC; Benzyloxycarbonyl-Val-Ala-Asn-7-Amino-4-methylcoumarin; Cbz-Val-Ala-Asn-AMC; Legumain Substrate; Caspase Substrate; Fluorogenic Peptide Substrate VAD-AMC (related); Z-VAN-AMC; Asparaginyl Endopeptidase Substrate
EINECS	Contact for details

Quality Control

Our Z-Val-Ala-Asn-Amc is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to relevant standards for research-grade biochemicals, ensuring reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in legumain assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.