Description

Boc-Lys(Ac)-Amc CAS NO 233691-67-3 is a protected amino acid derivative, specifically an acetylated lysine residue with N-terminal Boc (tert-butoxycarbonyl) protection and a C-terminal AMC (7-amino-4-methylcoumarin) fluorophore. This compound is a critical building block and fluorogenic substrate in advanced biochemical and pharmaceutical research. It is primarily utilized by researchers and manufacturers in the fields of proteomics, enzymology, and high-throughput screening for studying protease activity and developing novel therapeutic agents.

Application

• Protease Substrate: Acts as a sensitive fluorogenic substrate for the study of trypsin-like serine proteases, including trypsin, plasmin, and thrombin.
• Biochemical Research: Used in enzyme kinetics assays to determine kinetic parameters (Km, Vmax, kcat) and inhibitor screening.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors for drug discovery.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for detecting and quantifying specific protease activities in clinical samples.
• Peptide Synthesis Intermediate: Used as a specialized, fluorophore-labeled amino acid derivative in solid-phase peptide synthesis (SPPS) for creating fluorescently tagged peptides.
• Cell Biology Studies: Applied in live-cell imaging and assays to monitor intracellular protease activity and localization.

Basic Information

Product Name	Boc-Lys(Ac)-Amc
CAS No.	233691-67-3
Molecular Formula	C₂₈H₄₀N₄O₇
Molecular Weight	544.64 g/mol
Synonyms	Nα-(tert-Butoxycarbonyl)-Nε-acetyl-L-lysine 7-amido-4-methylcoumarin; Boc-Lys(Ac)-AMC; N-Boc-N'-acetyl-L-lysine-7-amido-4-methylcoumarin; Ac-Lys(Boc)-AMC; BOC-LYS(AC)-AMC; L-Lysine, N2-[(1,1-dimethylethoxy)carbonyl]-N6-acetyl-, 7-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)amide; Fluorogenic substrate for trypsin; Boc-Lys(Ac)-7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Boc-Lys(Ac)-Amc is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific optical rotation verification. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all test results to ensure compliance with research and development specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, cool place. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Optical Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.