Description

Naphthol As-Bi-Phosphate Disodium Salt Heptahydrate CAS NO 207569-04-8 is a high-purity biochemical reagent primarily used as a substrate for alkaline phosphatase in enzyme-linked immunoassays and histochemical staining. This compound is critical for producing a visible, insoluble colored precipitate, enabling the detection and localization of specific antigens or enzymes in biological samples. It is an essential component for researchers and manufacturers in the fields of diagnostic kit production, life science research, and clinical pathology.

Application

• Primary substrate in ELISA (Enzyme-Linked Immunosorbent Assay) and other immunoassay diagnostic kits.
• Key reagent for alkaline phosphatase (ALP) detection in Western blotting and immunohistochemistry (IHC).
• Used in the development and manufacturing of in-vitro diagnostic (IVD) devices and clinical chemistry analyzers.
• Essential for histochemical staining in research and clinical pathology to visualize tissue morphology and enzyme activity.
• Component in molecular biology research for nucleic acid detection systems.
• Applied in biochemical research for studying phosphatase enzyme kinetics and inhibition.

Basic Information

Product Name	Naphthol As-Bi-Phosphate Disodium Salt Heptahydrate
CAS No.	207569-04-8
Molecular Formula	C23H19NO6P•2Na•7H2O
Molecular Weight	Approx. 605.36 g/mol (Anhydrous basis: 491.34 g/mol)
Synonyms	Naphthol AS-BI Phosphate Disodium Salt; Naphthol AS-BI Phosphoric Acid Sodium Salt; 3-Hydroxy-4-[(2-hydroxy-3-naphthoyl)amino]phenyl Dihydrogen Phosphate Disodium Salt; AS-BI Phosphate; Naphthol AS-BI Phosphate; Fast Blue RR Salt Substrate; Alkaline Phosphatase Substrate; ALP Substrate; 4-[(2-Hydroxy-3-naphthamido)phenyl] Phosphoric Acid Disodium Salt
EINECS	Contact for details

Quality Control

Our Naphthol As-Bi-Phosphate Disodium Salt is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	18.0 - 22.0% (Theoretical for Heptahydrate: ~20.8%)
Solubility	Clear, colorless solution in water
Heavy Metals	< 20 ppm
Performance (Enzymatic)	Yields intense, insoluble precipitate with Alkaline Phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.