Description

L-Arginine:Hcl (15N4) CAS NO 204633-95-4 is a stable isotope-labeled derivative of the essential amino acid L-Arginine, where four nitrogen atoms (15N4) are enriched with the stable isotope Nitrogen-15. This compound is a critical tool for advanced research, enabling precise metabolic pathway tracing, protein turnover studies, and quantitative proteomics via mass spectrometry. It is indispensable for scientists and manufacturers in pharmaceutical development, clinical diagnostics, and nutritional research who require the highest level of isotopic purity and chemical stability for accurate and reliable data.

Application

• Stable Isotope Labeling in Mass Spectrometry (MS): Serves as an internal standard for the absolute quantification of arginine and related metabolites in complex biological samples.
• Metabolic Flux Analysis (MFA): Used to trace and model nitrogen metabolism, urea cycle activity, and nitric oxide synthesis pathways in cell cultures, tissues, and whole organisms.
• Proteomics and Protein Dynamics Research: Incorporated into proteins via cell culture (SILAC) to study protein synthesis, degradation rates, and post-translational modifications.
• Pharmaceutical R&D: Employed in drug metabolism and pharmacokinetics (DMPK) studies to track the fate of drug molecules containing arginine moieties.
• Clinical Diagnostic Assay Development: A key component in calibrators and controls for LC-MS/MS assays measuring arginine, citrulline, or asymmetric dimethylarginine (ADMA) for diagnostic purposes.
• Nutritional Science Research: Investigates protein metabolism, amino acid requirements, and the impact of dietary interventions in both human and animal studies.

Basic Information

Product Name	L-Arginine:Hcl (15N4)
CAS No.	204633-95-4
Molecular Formula	C6H1515N4O2 • HCl
Molecular Weight	~ 222.66 (based on 15N enrichment)
Synonyms	(2S)-2-Amino-5-[(15N)carbamimidamido]pentanoic acid hydrochloride; L-Arginine-15N4 hydrochloride; Arg-15N4 HCl; (15N4)-L-Arginine monohydrochloride; Nitrogen-15 labeled L-Arginine HCl; L-[15N4]Arginine hydrochloride; Stable isotope labeled L-Arginine HCl
EINECS	Contact for details

Quality Control

Every batch of our L-Arginine:Hcl (15N4) is produced and analyzed under strict quality management protocols. We guarantee high chemical and isotopic purity, with comprehensive testing including NMR and mass spectrometry to confirm isotopic enrichment and positional integrity. Certificates of Analysis (COA) are provided, detailing specifications for assay, optical rotation, residual solvents, and heavy metals. Our quality system supports compliance with relevant standards for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
15N Isotopic Enrichment	≥ 98 atom % 15N
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Optical Rotation	+21.0° to +23.0° (c=8 in 6N HCl)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.