Description

Fmoc-Ile-Oh-15N CAS NO 204633-89-6 is a high-purity, isotopically labeled amino acid derivative where the nitrogen atom is enriched with the stable isotope Nitrogen-15 (15N). This compound is essential for advanced research and development in the fields of proteomics, metabolomics, and pharmaceutical discovery, where precise mass spectrometry analysis is required. It serves as a critical building block for the synthesis of isotopically labeled peptides, enabling accurate quantification and tracking of protein dynamics, interactions, and degradation. Researchers and manufacturers in pharmaceutical R&D, academic institutions, and contract research organizations (CROs) rely on this labeled reagent for its role in creating internal standards and conducting sophisticated tracer studies.

Application

• Synthesis of 15N-labeled peptides for use as internal standards in quantitative mass spectrometry (MS) and liquid chromatography-mass spectrometry (LC-MS).
• Proteomics research, facilitating the study of protein-protein interactions, post-translational modifications, and metabolic pathways through stable isotope labeling techniques (SILAC).
• Pharmaceutical development for the creation of isotopically labeled drug candidates to study absorption, distribution, metabolism, and excretion (ADME) properties.
• Biomarker discovery and validation, where labeled peptides are used for accurate and sensitive detection of disease markers in complex biological samples.
• Nuclear Magnetic Resonance (NMR) spectroscopy studies, as the 15N label provides a sensitive probe for investigating protein structure and dynamics.
• Metabolic flux analysis in biological systems to trace the incorporation of nitrogen into amino acids and other metabolites.

Basic Information

Product Name	Fmoc-Ile-Oh-15N
CAS No.	204633-89-6
Molecular Formula	C21H2215NNO4
Molecular Weight	369.41 g/mol (for 15N labeled form)
Synonyms	Fmoc-L-isoleucine-15N; N-(9-Fluorenylmethoxycarbonyl)-L-isoleucine-15N; (2S,3S)-2-[[(9H-Fluoren-9-ylmethoxy)carbonyl]-15N]amino]-3-methylpentanoic acid; Fmoc-Ile(15N)-OH; Fmoc-Ile-OH (15N); Nα-Fmoc-L-isoleucine-15N; Fmoc-Isoleucine-15N
EINECS	Contact for details

Quality Control

Our Fmoc-Ile-Oh-15N is manufactured under strict quality control protocols to ensure exceptional isotopic and chemical purity, which is critical for sensitive analytical applications. Each batch is analyzed to confirm isotopic enrichment (typically ≥98 atom % 15N) and chemical purity (typically ≥95% by HPLC). We provide comprehensive Certificates of Analysis (COA) that detail specifications for assay, chiral purity, and the absence of critical impurities. Our quality system is designed to support research requiring the highest levels of traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Isotopic Purity (15N)	≥ 98 atom %
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.