Description

Ac-Ala-Ala-Ser(Po3H2)-Pro-Arg-Pna is a chemically defined, phosphorylated peptide substrate designed for high-precision biochemical research and development. This compound is critical for studying and quantifying the activity of specific serine/threonine kinases and phosphatases, providing essential insights into cellular signaling pathways. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic manufacturers engaged in enzyme kinetics, drug discovery, and assay development.

Application

• Kinase Activity Assays: A specific and sensitive substrate for measuring the enzymatic activity of target kinases in high-throughput screening (HTS) platforms.
• Phosphatase Research: Used as a substrate to study the kinetics and inhibition of protein phosphatases, aiding in the identification of novel therapeutic targets.
• Drug Discovery & Development: Integral in validating lead compounds and optimizing drug candidates that modulate phosphorylation-dependent signaling cascades.
• Diagnostic Kit Development: Serves as a key component in the formulation of ELISA and other immunoassay kits for clinical and research diagnostics.
• Biochemical Research: Employed in fundamental research to elucidate the role of specific phosphorylation events in cell biology, oncology, and neuroscience.
• Quality Control Reagent: Used as a reference standard or control in the quality assurance of enzyme preparations and assay systems.

Basic Information

Product Name	Ac-Ala-Ala-Ser(Po3H2)-Pro-Arg-Pna
CAS No.	202739-37-5
Molecular Formula	C27H43N11O12P
Molecular Weight	744.68 g/mol
Synonyms	Acetyl-Ala-Ala-phosphoSer-Pro-Arg-p-nitroanilide; Ac-A-A-S(P)-P-R-pNA; Phosphopeptide Substrate; pNA Substrate; Kinase Substrate Peptide; Phosphorylated Peptide pNA; Ac-Ala-Ala-Ser(PO3H2)-Pro-Arg-4-Nitroanilide; N-Acetyl-L-alanyl-L-alanyl-O-phosphono-L-seryl-L-prolyl-L-arginine 4-nitroanilide
EINECS	Contact for details

Quality Control

Our Ac-Ala-Ala-Ser(Po3H2)-Pro-Arg-Pna is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and NMR for structural verification, to ensure it meets the exacting standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, water content, and specific performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.