Description

Dabcyl-γ-Abu-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Glu(Edans)-Ala-Lys-Nh2 is a specialized, high-purity peptide substrate designed for sensitive protease activity assays. This compound matters because it incorporates a FRET (Förster Resonance Energy Transfer) pair, enabling precise, real-time, and quantitative measurement of enzymatic activity without the need for secondary detection steps. It is essential for researchers and quality control laboratories in the pharmaceutical and biotechnology sectors who require reliable tools for drug discovery, enzyme characterization, and diagnostic development. The product is supplied with a Certificate of Analysis to ensure batch-to-batch consistency and performance reliability.

Application

• Protease Activity Assays: Primary substrate for fluorometric detection of specific protease activity in high-throughput screening (HTS) platforms.
• Drug Discovery & Development: Used to screen and characterize potential therapeutic inhibitors for proteases involved in diseases.
• Biochemical Research: A critical tool for studying enzyme kinetics, specificity, and catalytic mechanisms in academic and industrial R&D.
• Diagnostic Kit Manufacturing: Serves as a key component in the development of clinical diagnostic assays for detecting protease-related biomarkers.
• Quality Control (QC) Testing: Employed in biopharmaceutical manufacturing to monitor the presence and activity of proteases during production and purification processes.

Basic Information

Product Name	Dabcyl-γ-Abu-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Glu(Edans)-Ala-Lys-Nh2
CAS No.	202273-56-1
Molecular Formula	C₁₀₀H₁₄₈N₃₂O₂₅
Molecular Weight	2263.5 g/mol (theoretical)
Synonyms	FRET Peptide Substrate; Dabcyl-γ-Abu-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Glu(Edans)-Ala-Lys-NH₂; EDANS/Dabcyl FRET Peptide; Cathepsin D Substrate; HIV-1 Protease Substrate; Fluorogenic Peptide Substrate; Dabcyl-GABA-Arg-Pro-Lys-Pro-Val-Glu-Norval-Trp-Arg-Glu(Edans)-Ala-Lys-amide
EINECS	Contact for details

Quality Control

Every batch of this FRET peptide substrate is manufactured under controlled conditions and undergoes comprehensive analytical testing to ensure it meets the stringent requirements of research and diagnostic applications. Quality is verified by HPLC for purity, mass spectrometry for identity confirmation, and functional activity assays to guarantee performance. A Certificate of Analysis (COA) detailing purity, net peptide content, and performance data is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Net Peptide Content	≥ 80%
Water Content (KF)	≤ 5.0%
Functional Activity	Conforms to reference (Specific activity provided on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.