Description

Fmoc-Leu-OH (C1-13C) is a high-purity, isotopically labeled derivative of the amino acid leucine, specifically enriched with carbon-13 at the C1 (carboxyl) position. This compound is essential for advanced research in proteomics and metabolic pathway analysis, where precise isotopic tracing is required. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and CROs specializing in peptide synthesis and mass spectrometry-based studies.

Application

• Isotope-Labeled Peptide Synthesis: As a stable isotope-labeled building block for the solid-phase synthesis of peptides for use as internal standards in quantitative mass spectrometry (MS).
• Metabolic Flux Analysis: Used in tracer studies to investigate cellular metabolism, protein turnover rates, and biosynthetic pathways in biological systems.
• Proteomics Research: Enables accurate quantification and identification of proteins and peptides in complex biological samples via techniques like SILAC (Stable Isotope Labeling by Amino acids in Cell culture).
• Pharmaceutical Development: Critical for the research and development of novel peptide-based therapeutics, where isotopic labeling aids in pharmacokinetic and ADME studies.
• NMR Spectroscopy: Serves as a labeled precursor for the preparation of samples for nuclear magnetic resonance (NMR) structural analysis of peptides and proteins.
• Biomarker Discovery: Facilitates the high-precision comparative analysis required for discovering and validating disease biomarkers.

Basic Information

Product Name	Fmoc-Leu-OH (C1-13C)
CAS No.	202114-53-2
Molecular Formula	C22H2413CNO4
Molecular Weight	~383.9 g/mol (for 13C isotopologue)
Synonyms	Fmoc-L-leucine-1-13C; (S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-4-methyl-1-13C-pentanoic acid; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-leucine-1-13C; Fmoc-Leu-1-13C-OH; Fmoc-L-Leu-1-13C; Nα-Fmoc-L-leucine-1-13C; 9-Fluorenylmethyloxycarbonyl-L-leucine-1-13C; Fmoc-13C1-Leu-OH
EINECS	Contact for details

Quality Control

Our Fmoc-Leu-OH (C1-13C) is manufactured under strict quality protocols to ensure the highest isotopic and chemical purity for sensitive research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically ≥99 atom % 13C), chemical purity (by HPLC), and confirming identity and absence of critical impurities. We adhere to cGMP principles where applicable to support our clients in regulated development environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥98.0%
13C Isotopic Enrichment	≥99 atom %
Specific Rotation	-15.0° to -25.0° (c=1 in DMF)
Water Content (KF)	≤0.5%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.