Description

Sulfosuccinimidyl 3-[[2-(Biotinamido)Ethyl] Dithio]Propionate is a heterobifunctional crosslinking reagent designed for the bioconjugation of biomolecules. Its core value lies in its dual functionality, featuring an amine-reactive NHS ester and a cleavable disulfide bond linked to a biotin moiety, enabling reversible labeling and purification. This compound is essential for researchers and manufacturers in biotechnology, pharmaceutical development, and diagnostic assay production who require precise protein modification, affinity capture, and controlled release systems.

Application

• Affinity Chromatography Probe Synthesis: For immobilizing proteins or peptides onto avidin/streptavidin resins.
• Reversible Protein Biotinylation: Enabling the capture and subsequent elution of target proteins under mild reducing conditions.
• Diagnostic Assay Development: Conjugation of antibodies or antigens with biotin for use in ELISA, lateral flow, or other immunoassay formats.
• Cell Surface Labeling: Tagging membrane proteins for isolation, tracking, or imaging studies.
• Drug Delivery System Conjugation: Linking therapeutic agents to targeting ligands via a cleavable linker.
• Pull-Down Assays: Preparation of biotinylated bait molecules for protein-protein interaction studies.

Basic Information

Product Name	Sulfosuccinimidyl 3-[[2-(Biotinamido)Ethyl] Dithio]Propionate
CAS No.	202057-28-1
Molecular Formula	C20H30N4O8S3
Molecular Weight	550.67 g/mol
Synonyms	Sulfo-NHS-SS-Biotin; NHS-SS-Biotin (sulfonated); N-Sulfosuccinimidyl 3-((2-(biotinamido)ethyl)dithio)propionate; Biotin-HPDP (sulfonated); 3-((2-(Biotinamido)ethyl)dithio)propanoic acid N-hydroxysulfosuccinimide ester; Sulfo-LC-SPDP-Biotin; Biotinylation Reagent with Cleavable Linker; EZ-Link Sulfo-NHS-SS-Biotin
EINECS	Contact for details

Quality Control

Our Sulfosuccinimidyl 3-[[2-(Biotinamido)Ethyl] Dithio]Propionate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional suitability. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards of research and diagnostic manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Functional Activity	Conforms (by biotinylation assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.