Description

Z-Gly-Ala-His-βna CAS NO 202001-31-8 is a synthetic tripeptide derivative, specifically a benzylamide, designed for advanced biochemical and pharmaceutical research. This compound is a crucial building block and research tool for studying enzyme-substrate interactions, particularly with proteases like matrix metalloproteinases (MMPs). It is essential for researchers and developers in pharmaceutical R&D, proteomics, and peptide synthesis who require high-purity, well-characterized substrates for assay development and inhibitor screening.

Application

• Protease Substrate: Used as a selective fluorogenic or colorimetric substrate for studying the activity and kinetics of matrix metalloproteinases (MMPs) and other zinc-dependent metalloproteases.
• Drug Discovery Research: Serves as a key biochemical tool in high-throughput screening (HTS) assays to identify and characterize novel protease inhibitors for therapeutic development.
• Enzyme Mechanism Studies: Employed in academic and industrial laboratories to investigate enzyme specificity, catalytic mechanisms, and structure-activity relationships (SAR).
• Biomarker Assay Development: Utilized in the development of diagnostic assays that measure specific protease activity in biological samples, relevant for cancer and inflammatory disease research.
• Peptide Chemistry Intermediate: Acts as a protected peptide fragment for the solid-phase or solution-phase synthesis of more complex peptide constructs.
• Biochemical Reagent: Supplied as a critical component in research kits focused on protease activity and inhibition.

Basic Information

Product Name	Z-Gly-Ala-His-βna
CAS No.	202001-31-8
Molecular Formula	C27H30N6O6
Molecular Weight	534.56 g/mol
Synonyms	Z-Gly-Ala-His-BNA; Benzyloxycarbonyl-Glycyl-Alanyl-Histidyl-Benzylamide; N-CBZ-Gly-Ala-His-Bzl; MMP Substrate (Fluorogenic); CBZ-Gly-Ala-His-BNA; (S)-2-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)acetamido)-N-(1H-imidazol-4-yl)methyl)propanamide (related); Gly-Ala-His peptide derivative; Protease substrate 202001-31-8
EINECS	Contact for details

Quality Control

Every batch of Z-Gly-Ala-His-βna is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our standard grade meets specifications suitable for research and development applications. Certificates of Analysis (COA) detailing purity, HPLC chromatograms, and other critical parameters are provided with each shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.