Description

H-Ala-Pro-Ala-βna CAS NO 201985-58-2 is a synthetic tripeptide derivative, specifically designed for advanced research and development applications. This compound is valued for its role as a specialized building block and biochemical probe in the study of peptide interactions and enzyme specificity. It is primarily utilized by pharmaceutical researchers, biotechnology companies, and academic institutions engaged in drug discovery, proteomics, and the development of novel therapeutic agents.

Application

• Pharmaceutical Research & Development: Serves as a key intermediate or reference standard in the synthesis and screening of new peptide-based drug candidates.
• Protease/Enzyme Substrate Studies: Used as a specific substrate to investigate the activity, inhibition, and kinetics of proteolytic enzymes like dipeptidyl peptidases (DPP).
• Biochemical Assay Development: Employed in the design and validation of high-throughput screening (HTS) assays and diagnostic kits.
• Academic Research: A crucial tool for studying peptide structure-activity relationships (SAR), metabolic pathways, and signal transduction mechanisms.
• Peptide Library Synthesis: Acts as a building block for constructing diverse peptide libraries used in combinatorial chemistry and lead optimization.

Basic Information

Product Name	H-Ala-Pro-Ala-βna
CAS No.	201985-58-2
Molecular Formula	C₂₂H₂₈N₄O₅
Molecular Weight	428.48 g/mol
Synonyms	Ala-Pro-Ala-4-nitroanilide; H-Ala-Pro-Ala-pNA; L-Alanyl-L-prolyl-L-alanine 4-nitroanilide; Ala-Pro-Ala-p-Nitroanilide; APApNA; DPP Substrate; Peptide substrate for DPP-IV; N-(3-(1-((S)-1-((S)-2-Aminopropanamido)prolyl)amino)-1-oxopropan-2-yl)amino)-3-oxopropyl)-4-nitrobenzenesulfonamide (related IUPAC form)
EINECS	Contact for details

Quality Control

Every batch of H-Ala-Pro-Ala-βna is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our standard quality protocol includes HPLC for purity determination, mass spectrometry (MS) for confirmation, and comprehensive characterization. A Certificate of Analysis (COA) detailing all test results is provided with each shipment to support your quality assurance and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	< 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.