Description

Z-Gly-Ala-His-Amc is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise measurement of protease activity, particularly dipeptidyl peptidase IV (DPP-IV) and related enzymes. It is primarily utilized in pharmaceutical R&D, biochemical research, and diagnostic kit development for studying enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous, real-time monitoring of DPP-IV (CD26) enzyme activity.
• Pharmaceutical Research: High-throughput screening (HTS) for identifying and characterizing novel DPP-IV inhibitors, a major target for type 2 diabetes therapeutics.
• Biochemical Research: Kinetic studies and mechanistic analysis of serine proteases and aminopeptidases in various biological pathways.
• Diagnostic Development: A key component in the formulation of clinical diagnostic kits for measuring serum DPP-IV levels.
• Cell Biology Studies: Investigating the role of DPP-IV in immune regulation, cancer biology, and signal transduction.
• Quality Control: Used as a reference standard in the QC of biopharmaceuticals where protease activity must be controlled.

Basic Information

Product Name	Z-Gly-Ala-His-Amc
CAS No.	201928-37-2
Molecular Formula	C26H30N6O7
Molecular Weight	538.55 g/mol
Synonyms	Z-Gly-Ala-His-AMC; Z-Gly-Ala-His-7-amido-4-methylcoumarin; Benzyloxycarbonyl-Gly-Ala-His-AMC; DPP-IV Substrate (Z-Gly-Ala-His-AMC); H-Gly-Ala-His-AMC, Nα-Z-protected; Fluorogenic DPP-IV Substrate; CD26 Fluorogenic Peptide Substrate; Z-G-A-H-AMC
EINECS	Contact for details

Quality Control

Our Z-Gly-Ala-His-Amc is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. We support compliance with relevant guidelines for research-use materials.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity	≥ 90% of reference standard in DPP-IV assay
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.