Description

Suc-Tyr-Val-Ala-Asp-Amc CAS NO 201860-29-9 is a fluorogenic peptide substrate designed for the continuous assay of caspase-1 (ICE) and related cysteine proteases. This compound is critical for biochemical research and drug discovery, enabling precise measurement of enzymatic activity through the release of the fluorescent 7-amino-4-methylcoumarin (AMC) group upon cleavage. It is an essential tool for researchers in pharmaceutical development, academic biochemistry, and proteomics studying apoptosis, inflammation, and other caspase-mediated pathways.

Application

• Caspase-1 (Interleukin-1β Converting Enzyme, ICE) Activity Assays: Primary fluorogenic substrate for the specific and sensitive detection of caspase-1 enzymatic activity in vitro.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel inhibitors or activators of caspase-1 for therapeutic development.
• Apoptosis and Inflammation Research: A key reagent for studying the role of caspase-1 in pyroptosis (inflammatory cell death) and the maturation of pro-inflammatory cytokines like IL-1β.
• Enzyme Kinetics and Characterization: Employed to determine kinetic parameters (Km, Vmax, kcat) and study the specificity of caspase-1 and related proteases.
• Cell-Based Assays: Can be used in cell lysates or with purified enzymes to monitor caspase activation in response to various stimuli.
• Biomarker Development: Supports research into caspase-1 activity as a potential biomarker for inflammatory and autoimmune diseases.

Basic Information

Product Name	Suc-Tyr-Val-Ala-Asp-Amc
CAS No.	201860-29-9
Molecular Formula	C36H45N7O13
Molecular Weight	783.79 g/mol
Synonyms	Succinyl-Tyr-Val-Ala-Asp-7-Amino-4-methylcoumarin; Suc-YVAD-AMC; Caspase-1 Substrate IV; ICE Substrate; Ac-YVAD-AMC (common misnomer); Suc-Tyr-Val-Ala-Asp-AMC; Fluorogenic Caspase-1 Substrate; N-Succinyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Our Suc-Tyr-Val-Ala-Asp-Amc is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency for research reproducibility. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in functional assays. We adhere to ISO 9001 quality management standards, and our production environment is designed to meet the rigorous demands of life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO or DMF
Functional Activity	Cleavage by recombinant caspase-1 confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.