Description

H-Phe-Pro-Ala-Pna CAS NO 201738-99-0 is a synthetic peptide sequence containing phenylalanine, proline, and alanine, capped with a para-nitroanilide (Pna) group. This compound is primarily valued as a specialized chromogenic substrate for the study and characterization of protease enzyme activity, particularly in biochemical and pharmaceutical research. It is an essential tool for researchers and quality control laboratories in the life sciences, biotechnology, and pharmaceutical development sectors who require precise, reliable substrates for enzymatic assays.

Application

• Protease Enzyme Assays: Acts as a chromogenic substrate for kinetic studies and activity measurements of various proteolytic enzymes, including dipeptidyl peptidases and other serine or cysteine proteases.
• Biochemical Research: Used in fundamental research to investigate enzyme mechanisms, inhibition profiles, and substrate specificity in vitro.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitor drug candidates.
• Diagnostic Reagent Development: Serves as a key component in the development of diagnostic kits and reagents for detecting specific protease activities associated with diseases.
• Quality Control in Biomanufacturing: Utilized to monitor and validate the activity and consistency of enzyme batches used in biopharmaceutical production processes.

Basic Information

Item	Detail
Product Name	H-Phe-Pro-Ala-Pna
CAS No.	201738-99-0
Molecular Formula	C23H27N5O5
Molecular Weight	453.49 g/mol
Synonyms	H-Phe-Pro-Ala-pNA; Phe-Pro-Ala-p-Nitroanilide; DPP IV Substrate (Phe-Pro-Ala-pNA); Gly-Pro-pNA (related); H-Phe-Pro-Ala-4-nitroanilide; Substrate for Dipeptidyl Peptidase; Chromogenic Peptide Substrate; 4-Nitroanilide of H-Phe-Pro-Ala
EINECS	Contact for details

Quality Control

Our H-Phe-Pro-Ala-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, identity confirmation by mass spectrometry (MS) and infrared (IR) spectroscopy, and precise quantification of water content. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.