Description

Dl-Dithiothreitol (DTT) is a powerful, low-molecular-weight reducing agent widely used to cleave disulfide bonds within and between biological molecules. Its primary commercial value lies in its ability to maintain proteins and enzymes in a reduced, active state, which is critical for numerous biochemical and biotechnological processes. This compound is essential for industries including pharmaceutical development, molecular biology research, and diagnostic kit manufacturing, where precise control over protein structure is required.

Application

• Protein Biochemistry: Reduction and denaturation of proteins prior to SDS-PAGE analysis.
• Enzyme Activity Studies: Maintaining sulfhydryl-dependent enzymes in their active, reduced form.
• Nucleic Acid Research: Stabilization of reagents and prevention of oxidative damage in PCR and other amplification techniques.
• Pharmaceutical Manufacturing: Used in the production and purification of therapeutic proteins and monoclonal antibodies.
• Diagnostic Assays: Component in lateral flow assays and ELISA kits to ensure consistent reagent performance.
• Cell Culture: Supplement in certain media formulations to reduce oxidative stress.

Basic Information

Product Name	Dl-Dithiothreitol
CAS No.	27565-41-9
Molecular Formula	C4H10O2S2
Molecular Weight	154.25 g/mol
Synonyms	DTT; DL-Dithiothreitol; (±)-1,4-Dithiothreitol; Cleland's Reagent; Dithioerythritol; DTE; 2,3-Dihydroxy-1,4-butanedithiol; Threo-1,4-dimercapto-2,3-butanediol
EINECS	248-534-8

Quality Control

Our Dl-Dithiothreitol is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure high purity and consistency, meeting the requirements for research and industrial applications. A Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) to minimize oxidation and moisture absorption. Allow the container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	40 - 44 °C
Water Content (KF)	≤ 2.0 %
Optical Rotation	-0.10° to +0.10°

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.