Description

Poly-DL-Ornithine Hydrobromide is a synthetic polycationic amino acid polymer widely used as a coating agent to enhance cell adhesion in biological research. This compound is critical for creating a positively charged surface that facilitates the attachment of cells, particularly those that are difficult to culture. It is an essential reagent for researchers and manufacturers in the life sciences, biotechnology, and pharmaceutical development sectors.

Application

• Cell Culture Substrate Coating: Used to coat tissue culture plates, flasks, and coverslips to promote the adhesion of neurons, endothelial cells, and other anchorage-dependent cells.
• Neuroscience Research: Essential for the culture of primary neurons and glial cells, supporting studies in neurodevelopment, neurotoxicity, and synaptic function.
• Biomedical Material Functionalization: Applied to modify the surface of biomaterials, scaffolds, and medical devices to improve biocompatibility and cellular integration.
• Stem Cell Research: Facilitates the attachment and expansion of various stem cell types in defined culture systems.
• Virus and Gene Delivery Studies: Serves as a transduction enhancer in protocols involving lentiviral or adenoviral gene delivery by increasing viral particle attachment to target cells.
• In Vitro Diagnostics (IVD): Used in the development of diagnostic assays that require immobilized cells for high-content screening or toxicity testing.

Basic Information

Product Name	Poly-DL-Ornithine Hydrobromide
CAS No.	26445-53-4
Molecular Formula	(C5H12N2O2 • HBr)n
Molecular Weight	~15,000 - 70,000 (Average MW, varies by grade)
Synonyms	Poly(ornithine) hydrobromide; PLO; Poly-DL-ornithine HBr; DL-Polyornithine hydrobromide; Ornithine polymer hydrobromide; Polyornithine hydrobromide salt; NSC 14699; Poly(ornithine hydrobromide)
EINECS	Contact for details

Quality Control

Our Poly-DL-Ornithine Hydrobromide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent molecular weight distribution, and optimal biological performance. Certificates of Analysis (COA) detailing specifications such as assay, endotoxin levels, and sterility (where applicable) are provided to support research and GLP/GMP compliance.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (IR)	Conforms to structure
Assay (Nitrogen)	≥ 95.0%
Molecular Weight (Average)	15,000 - 70,000 Da (Specific range available by grade)
pH (1% solution)	5.0 - 7.0
Endotoxin	< 1.0 EU/mg (for cell culture grade)
Solubility	Soluble in water, phosphate buffered saline (PBS)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.