Description

S-(2,4-Dinitrophenyl)-Glutathione is a chemically modified derivative of the tripeptide glutathione, where a 2,4-dinitrophenyl group is attached via a thioether bond to the cysteine sulfur. This compound matters as a critical biochemical tool and reference standard for studying glutathione S-transferase (GST) enzyme activity, a key detoxification pathway. It is primarily needed by research institutions, pharmaceutical R&D departments, and diagnostic manufacturers focused on enzymology, toxicology, and oxidative stress research.

Application

• Biochemical Research: As a standard substrate for the spectrophotometric assay of glutathione S-transferase (GST) activity.
• Enzyme Kinetics Studies: Used to determine kinetic parameters (Km, Vmax) and inhibition profiles of GST isoenzymes.
• Toxicology Screening: Employed in in vitro models to assess the detoxification capacity of cells and tissues.
• Diagnostic Development: Serves as a key component in developing clinical diagnostic kits for liver function and metabolic disorders.
• Pharmaceutical R&D: Used to screen and evaluate potential drug candidates that modulate GST activity.
• Reference Standard: Acts as a high-purity calibrant for analytical methods (HPLC, LC-MS) quantifying glutathione conjugates.

Basic Information

Product Name	S-(2,4-Dinitrophenyl)-Glutathione
CAS No.	26289-39-4
Molecular Formula	C₁₆H₂₀N₄O₁₀S
Molecular Weight	460.42 g/mol
Synonyms	S-(2,4-Dinitrophenyl)glutathione; 2,4-Dinitrophenyl Glutathione; DNP-GSH; Glutathione S-(2,4-dinitrophenyl) ether; γ-Glu-Cys-Gly-S-DNP; S-(2,4-Dinitrophenyl)-L-glutathione; (2S)-2-Amino-4-{[(1R)-1-[(carboxymethyl)carbamoyl]-2-[(2,4-dinitrophenyl)sulfanyl]ethyl]carbamoyl}butanoic acid
EINECS	Contact for details

Quality Control

Our S-(2,4-Dinitrophenyl)-Glutathione is manufactured under strict quality protocols suitable for research and diagnostic applications. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) is provided, detailing results from HPLC, NMR, and mass spectrometry. We support compliance with relevant Good Laboratory Practice (GLP) standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage, consider desiccant and under inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, aqueous buffer (pH ~7.4)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.