Description

2-Maleimido Acetic Acid is a heterobifunctional crosslinking agent featuring a maleimide group and a carboxylic acid group. This molecular structure is critical for creating stable, site-specific conjugations between thiol-containing molecules and amine-containing molecules under mild conditions. It is an essential building block for researchers and manufacturers in the bioconjugation and pharmaceutical fields who require precise and reliable protein modification, antibody-drug conjugate (ADC) development, and surface functionalization.

Application

• Bioconjugation & Protein Labeling: Site-specific coupling of peptides, proteins, or antibodies to enzymes, fluorophores, or other biomolecules via thiol-maleimide chemistry.
• Antibody-Drug Conjugate (ADC) Linker Precursor: Serves as a key intermediate in the synthesis of cleavable or non-cleavable linkers for targeted cancer therapies.
• Surface Functionalization: Modification of gold nanoparticles, biosensor chips, or chromatographic supports to create reactive surfaces for biomolecule immobilization.
• Polymer & Material Science: Incorporation into polymer backbones or hydrogels to introduce reactive handles for further chemical modification or crosslinking.
• Diagnostic Reagent Synthesis: Used in the preparation of enzyme-linked immunosorbent assay (ELISA) components and other diagnostic probes.
• Peptide & Small Molecule Conjugation: Facilitating the attachment of functional payloads, such as drugs or imaging agents, to targeting ligands.

Basic Information

Product Name	2-Maleimido Acetic Acid
CAS No.	25021-08-3
Molecular Formula	C6H5NO4
Molecular Weight	155.11 g/mol
Synonyms	Maleimidoglycine; N-(Carboxymethyl)maleimide; 2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)acetic Acid; Maleimidoacetic Acid; 1H-Pyrrole-1-acetic acid, 2,5-dihydro-2,5-dioxo-; Glycine, N-maleoyl-; (Maleimido)acetic Acid; Maleimido Acetate
EINECS	Contact for details

Quality Control

Our 2-Maleimido Acetic Acid is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and biopharmaceutical applications. Certificates of Analysis (COA) with detailed specifications are provided to guarantee traceability, consistency, and reliability for your critical workflows.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	158 - 162 °C
Loss on Drying	≤ 0.5%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.