Description

H-Pro-Pro-Gly-Phe-Ser-Pro-OH is a protected hexapeptide with defined sequence and stereochemical integrity, widely employed as a biochemical reference standard and synthetic intermediate in peptide-based drug discovery. Its precisely ordered structure enables reliable use in structure-activity relationship (SAR) studies, enzyme substrate profiling, and immunoassay development—ensuring reproducibility across R&D and QC laboratories. This compound is essential for pharmaceutical researchers, diagnostic reagent manufacturers, and contract research organizations (CROs) engaged in neuropeptide and protease-targeted therapeutic programs. H-Pro-Pro-Gly-Phe-Ser-Pro-Oh CAS NO 23828-06-0 is supplied under stringent quality controls to meet global regulatory expectations for analytical-grade peptides.

Application

• Substrate for prolyl endopeptidase (PEP) and other proline-specific proteases in enzymatic activity assays
• Reference standard in HPLC and LC-MS method validation for peptide quantification and purity assessment
• Synthetic building block for extended neuroactive peptides, including analogs of vasopressin and oxytocin
• Calibration material in clinical diagnostics for detecting proteolytic biomarkers in neurological disorders
• Tool compound in high-throughput screening (HTS) platforms targeting serine proteases and peptidyl-prolyl isomerases
• Component in custom peptide conjugate development for antibody-drug conjugates (ADCs) and targeted delivery systems

Basic Information

Product Name	H-Pro-Pro-Gly-Phe-Ser-Pro-OH
CAS No.	23828-06-0
Molecular Formula	C33H45N7O10
Molecular Weight	715.76 g/mol
Synonyms	H-Pro-Pro-Gly-Phe-Ser-Pro-OH; Pro-Pro-Gly-Phe-Ser-Pro-OH; Boc-Pro-Pro-Gly-Phe-Ser-Pro-OH (deprotected form); L-Pro-L-Pro-Gly-L-Phe-L-Ser-L-Pro-OH; H-D-Pro-D-Pro-Gly-Phe-Ser-Pro-OH (note: natural L-configuration assumed unless specified); Hexapeptide PPSFSP; PP-GFS-P-OH; Peptide PPSFSP-OH; Prolyl-Prolyl-Glycyl-Phenylalanyl-Seryl-Proline
EINECS	Not listed (no EINECS number assigned)

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for identity (IR, NMR, MS), assay (HPLC), enantiomeric purity (chiral HPLC), residual solvents (GC), and heavy metals (ICP-MS). The product conforms to ACS Reagent Grade and EP/USP general chapter <231> requirements for peptide reference materials. Full traceability and GMP-aligned documentation support regulatory submissions in FDA, EMA, and PMDA jurisdictions.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent oxidation and moisture uptake. Due to its hygroscopic nature, minimize exposure to ambient humidity during handling. Maintain desiccated conditions (<30% RH) during long-term storage.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identity (IR)	Conforms to reference spectrum
Identity (MS)	[M+H]+ m/z = 716.3 ± 0.5
Assay (HPLC)	≥98.5%
Single Impurity (HPLC)	≤0.5%
Total Impurities (HPLC)	≤1.5%
Residual Solvents (GC)	MeOH ≤ 3000 ppm; Acetonitrile ≤ 410 ppm; DCM ≤ 600 ppm
Water Content (KF)	≤5.0%
Heavy Metals (ICP-MS)	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.