Description

5'-o-(Triphenylmethyl)Cytidine is a protected nucleoside derivative where the 5'-hydroxyl group of cytidine is blocked by a trityl (triphenylmethyl) group. This chemical modification is critical for the controlled synthesis of oligonucleotides, preventing unwanted side reactions during phosphoramidite-based coupling steps. It is an essential building block for researchers and manufacturers in the pharmaceutical and biotechnology sectors developing nucleic acid-based therapeutics, diagnostics, and research tools. The compound, identified by CAS No. 22596-01-6, is supplied to meet the stringent purity requirements of advanced R&D and cGMP production environments.

Application

• Oligonucleotide Synthesis: Serves as a key protected intermediate in solid-phase and solution-phase synthesis of DNA and RNA strands.
• Antisense & siRNA Therapeutics: Used in the manufacturing of active pharmaceutical ingredients (APIs) for gene-silencing drugs.
• PCR Primers & Probes: Enables the production of high-purity primers and fluorescent probes for molecular diagnostics.
• Research Chemicals: A vital reagent for academic and industrial research in molecular biology, genetics, and biochemistry.
• Phosphoramidite Production: Acts as a precursor for the synthesis of cytidine phosphoramidites, the monomers used in automated DNA synthesis.
• Nucleoside Analogue Development: Utilized in medicinal chemistry for creating novel nucleoside-based antiviral and anticancer agents.

Basic Information

Product Name	5'-o-(Triphenylmethyl)Cytidine
CAS No.	22596-01-6
Molecular Formula	C28H27N3O5
Molecular Weight	485.53 g/mol
Synonyms	5'-O-Tritylcytidine; N4-Benzoyl-5'-O-tritylcytidine (common protected form); 1-[(2R,3R,4R,5R)-5-[[Tris(phenyl)methyl]oxymethyl]-3,4-dihydroxyoxolan-2-yl]-4-aminopyrimidin-2-one; Cytidine, 5'-O-(triphenylmethyl)-; Tritylcytidine; 5'-O-(Triphenylmethyl)cytidine; 5'-O-Trityl-Cytidine
EINECS	Contact for details

Quality Control

Our 5'-o-(Triphenylmethyl)Cytidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and tests for residual solvents and moisture content. We provide full traceability and Certificates of Analysis (COA) are available for every shipment, ensuring compliance with the rigorous standards required for pharmaceutical intermediates and advanced research applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.