Description

L-Leucine-[3,4,5-3H(N)] is a high-specific-activity, tritium-labeled isotopologue of the essential branched-chain amino acid L-Leucine. This labeled compound is a critical tool for metabolic and pharmacokinetic studies, enabling precise tracking and quantification in biological systems. It is primarily utilized by researchers and scientists in the pharmaceutical, biotechnology, and academic sectors for advanced tracer applications, drug metabolism research, and proteomic studies.

Application

• Metabolic Pathway Tracing: Used as a radiolabeled tracer to study protein synthesis rates, amino acid metabolism, and turnover in cell cultures, tissues, and whole organisms.
• Pharmacokinetics & Drug Metabolism (DMPK): Essential for Absorption, Distribution, Metabolism, and Excretion (ADME) studies to track the fate of leucine-containing drug candidates or nutrients.
• Proteomics Research: Employed in Stable Isotope Labeling by Amino acids in Cell culture (SILAC) and other quantitative proteomic techniques for accurate protein quantification.
• Biomedical Research: Investigates the role of leucine in muscle protein synthesis, metabolic disorders (e.g., Maple Syrup Urine Disease), and signaling pathways such as mTOR activation.
• Diagnostic Agent Development: Serves as a precursor for synthesizing more complex radiolabeled diagnostic probes for imaging techniques.

Basic Information

Product Name	L-Leucine-[3,4,5-3H(N)]
CAS No.	21675-61-6
Molecular Formula	C6H103H3NO2 *
Molecular Weight	~136.16 g/mol (for unlabeled C6H13NO2)
Synonyms	(2S)-2-Amino-4-methyl-[3,4,5-3H3
L-Leucine-t3
Tritiated L-Leucine
[3,4,5-3H]-L-Leucine
L-Leucine (ring-3,4,5-3H(N))
H-Leu-OH (tritiated)
(S)-2-Amino-4-methylpentanoic-3,4,5-3H3 acid
EINECS	Contact for details

* Formula indicates tritium (3H) labeling at the 3, 4, and 5 positions of the carbon chain. Exact molecular weight depends on specific activity.

Quality Control

Every batch of L-Leucine-[3,4,5-3H(N)] is manufactured and analyzed under strict quality systems to ensure the highest standards of radiochemical purity, chemical purity, and specific activity. Our quality commitment includes comprehensive analytical testing via HPLC and radiometric detection. A detailed Certificate of Analysis (COA), specifying specific activity (Ci/mmol or GBq/mmol), radiochemical purity, chemical purity, and solvent composition, is provided with each shipment to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at ≤ -20°C or as recommended on the specific product COA. Store in accordance with all local and national regulations for radioactive materials. Allow the product to reach ambient temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or solution (as specified)
Identification (IR / NMR)	Conforms to structure
Specific Activity	Customizable (e.g., 50-120 Ci/mmol)
Radiochemical Purity (HPLC)	≥ 95% to ≥ 98%
Chemical Purity (HPLC)	≥ 98%
Solvent (if supplied in solution)	Ethanol/Water or as specified
Radioactive Concentration	As per customer requirement (mCi/mL or MBq/mL)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.