Description

Z-L-Leucine-β-Naphthylamide Monohydrate is a specialized chromogenic substrate used in enzymatic assays and biochemical research. This compound is critical for the detection and quantification of specific protease activities, particularly leucine aminopeptidase (LAP). It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics studies, diagnostic development, and high-throughput screening.

Application

• Enzymatic Substrate for Leucine Aminopeptidase (LAP): Serves as a key chromogenic reagent for the colorimetric detection and kinetic analysis of LAP enzyme activity in biochemical assays.
• Diagnostic Reagent Manufacturing: Used in the formulation of clinical diagnostic kits for measuring LAP levels, which can be indicative of liver function and certain disease states.
• Biochemical Research & Protease Profiling: Essential for academic and industrial research focused on protease specificity, enzyme inhibition studies, and pathway analysis.
• Pharmaceutical Development: Employed in drug discovery platforms for screening potential inhibitors of aminopeptidases, which are targets for various therapeutic areas.
• Microbiology & Cell Biology: Applied in assays to study microbial enzyme production or cellular proteolytic processes.
• Quality Control in Biotechnology: Acts as a reference standard or assay component for monitoring the activity of enzyme preparations and bioprocesses.

Basic Information

Product Name	Z-L-Leucine-β-Naphthylamide Monohydrate
CAS No.	20998-86-1
Molecular Formula	C23H26N2O3 • H2O
Molecular Weight	396.47 g/mol (Monohydrate)
Synonyms	Z-Leu-β-NA; Z-Leu-β-Naphthylamide Monohydrate; N-Benzyloxycarbonyl-L-leucyl-β-naphthylamide Monohydrate; N-CBZ-L-Leucyl-β-naphthylamide Monohydrate; L-Leucine, N-[(phenylmethoxy)carbonyl]-, 1-(2-naphthalenyl)ethyl ester, monohydrate; Benzyl N-[(2S)-1-[(2-naphthyl)amino]-4-methyl-1-oxopentan-2-yl]carbamate monohydrate; CBZ-Leu-β-NA Monohydrate; Substrate for Leucine Aminopeptidase
EINECS	Contact for details

Quality Control

Our Z-L-Leucine-β-Naphthylamide Monohydrate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, identity, and performance consistency suitable for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity (typically >98% by HPLC), identification, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which may affect performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	4.0% - 6.0% (Theoretical for monohydrate: ~4.5%)
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm
Residue on Ignition	< 0.1%
Performance (Enzymatic)	Suitable for use as LAP substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.