Description

N(Alpha), n-(Im)-Di-Boc-L-Histidine Methyl Ester is a key protected amino acid derivative, specifically a Boc-protected histidine methyl ester, essential for advanced peptide synthesis. This compound provides critical orthogonal protection for the histidine side chain, enabling precise and efficient construction of complex peptides and peptidomimetics. It is primarily required by research institutions and pharmaceutical companies engaged in the development of therapeutic peptides, diagnostic agents, and biochemical tools.

Application

• Solid-Phase Peptide Synthesis (SPPS): A crucial building block for the controlled incorporation of histidine residues into peptide chains, particularly in Fmoc/t-Bu strategies.
• Pharmaceutical Intermediate: Used in the research and development of peptide-based drugs targeting various diseases, including metabolic disorders and oncology.
• Bioconjugation & Labeling: Serves as a precursor for creating histidine-tagged molecules or for site-specific modifications in protein engineering.
• Combinatorial Chemistry: Employed in libraries for high-throughput screening to discover new bioactive compounds.
• Academic & Industrial Research: Fundamental reagent for studying peptide structure-activity relationships (SAR) and developing novel biomaterials.

Basic Information

Product Name	N(Alpha), n-(Im)-Di-Boc-L-Histidine Methyl Ester
CAS No.	20898-43-5
Molecular Formula	C₂₂H₃₅N₃O₈
Molecular Weight	469.53 g/mol
Synonyms	Bis-Boc-L-Histidine Methyl Ester; Di-Boc-L-Histidine Methyl Ester; Nα,Nim-Bis(tert-butoxycarbonyl)-L-histidine methyl ester; L-Histidine, N-[N-[1,1-dimethylethoxy)carbonyl]-N-[(1,1-dimethylethoxy)carbonyl]-, methyl ester; Boc-His(Boc)-OMe; Methyl Nα,Nim-bis(tert-butoxycarbonyl)-L-histidinate; 20898-43-5
EINECS	Contact for details

Quality Control

Our N(Alpha), n-(Im)-Di-Boc-L-Histidine Methyl Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable to support our clients' regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Optical Rotation [α]²⁰D	Contact for details
Water Content (KF)	≤0.5%
Residual Solvents (GC)	Complies with ICH guidelines
Heavy Metals	<10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.