Description

H-Trp-Phe-Tyr-Ser(Po3H2)-Pro-Arg-Amc Trifluoroacetate Salt is a synthetic, phosphorylated peptide substrate designed for high-specificity enzymatic assays. This compound matters for its critical role in biochemical research, particularly in the study of kinase activity and signal transduction pathways. It is primarily needed by pharmaceutical R&D laboratories, academic research institutions, and diagnostic kit manufacturers focused on developing and validating assays for proteomics and drug discovery.

Application

• Biochemical Research: Used as a specific fluorogenic substrate for studying the kinetics and inhibition profiles of various serine/threonine and tyrosine kinases.
• Drug Discovery & Screening: Essential in high-throughput screening (HTS) platforms to identify and characterize potential kinase inhibitor drug candidates.
• Diagnostic Development: Employed in the development of in vitro diagnostic (IVD) assays to measure specific kinase activity in clinical samples.
• Enzyme Characterization: Facilitates the determination of kinetic parameters (Km, Vmax) and specificity constants for purified enzymes.
• Cell Signaling Pathway Analysis: A tool for investigating intracellular signaling cascades by measuring the activity of key regulatory kinases.
• Quality Control in Biopharma: Can be utilized in the quality control of enzyme-based biopharmaceutical products to ensure batch-to-batch consistency.

Basic Information

Product Name	H-Trp-Phe-Tyr-Ser(Po3H2)-Pro-Arg-Amc Trifluoroacetate Salt
CAS No.	1926163-51-0
Molecular Formula	C55H68N13O15P • xC2HF3O2
Molecular Weight	~1250.2 g/mol (Free base)
Synonyms	Phospho-Serine Peptide Substrate (Trp-Phe-Tyr-Ser(P)-Pro-Arg-AMC); Kinase Substrate Peptide AMC-conjugate; H-Trp-Phe-Tyr-phosphoSer-Pro-Arg-7-Amino-4-methylcoumarin TFA Salt; WFpYSPR-AMC TFA Salt; Fluorogenic Kinase Substrate 1926163-51-0; AMC-labeled Phosphopeptide; pSer-containing Fluorogenic Peptide
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Every batch is characterized using advanced analytical techniques including HPLC, MS, and NMR to confirm identity, purity, and correct phosphorylation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request, supporting research reproducibility and regulatory submissions.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum confirms molecular ion
Purity (HPLC)	≥ 95% (Area Percent)
Assay (HPLC)	≥ 90% (by weight)
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.