Description

4-(Azidomethyl)-L-Phenylalanine HCl is a non-canonical amino acid derivative featuring a reactive azidomethyl group on the phenylalanine aromatic ring. This compound is a crucial building block in chemical biology and pharmaceutical research, enabling site-specific bioconjugation via click chemistry reactions. It is primarily utilized by researchers and manufacturers in the fields of peptide synthesis, antibody-drug conjugate (ADC) development, and protein engineering to create novel, targeted therapeutics and diagnostic tools.

Application

• Antibody-Drug Conjugate (ADC) Linker-Payload Synthesis: Used for the site-specific incorporation of cytotoxic drugs onto monoclonal antibodies via strain-promoted azide-alkyne cycloaddition (SPAAC).
• Peptide & Protein Modification: Enables the post-translational or synthetic introduction of bioorthogonal handles into peptides and proteins for labeling, immobilization, or cross-linking.
• Chemical Biology Probes: Serves as a key reagent in the development of activity-based probes and affinity tags to study enzyme function and protein-protein interactions.
• Materials Science & Surface Functionalization: Used to modify surfaces, polymers, and nanomaterials with bioactive peptides or proteins for biosensor and tissue engineering applications.
• Radiopharmaceuticals: Acts as a precursor for the development of radiolabeled peptides for positron emission tomography (PET) imaging and targeted radiotherapy.
• Library Synthesis for Drug Discovery: Incorporated into combinatorial libraries to create diverse sets of compounds with unique chemical functionality for high-throughput screening.

Basic Information

Product Name	4-(Azidomethyl)-L-Phenylalanine HCl
CAS No.	1446772-80-0
Molecular Formula	C10H12N4O2 • HCl
Molecular Weight	256.69 g/mol
Synonyms	Fmoc-4-Azidomethyl-L-phenylalanine; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-4-(azidomethyl)-L-phenylalanine; 4-(Azidomethyl)-L-Phenylalanine hydrochloride; L-Phenylalanine, 4-(azidomethyl)-, hydrochloride; Azidomethyl-Phe-OH • HCl; p-Azidomethylphenylalanine HCl; Azi-Phe-OH • HCl; Click Chemistry Amino Acid Derivative
EINECS	Contact for details

Quality Control

Our 4-(Azidomethyl)-L-Phenylalanine HCl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis, and NMR for structural confirmation, to ensure it meets the high standards required for research and pre-clinical development. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.