Description

Azadibenzocyclooctyne-Biotin Conjugate is a high-purity, bifunctional chemical reagent designed for advanced bioorthogonal labeling and detection workflows. Its core value lies in combining the rapid, copper-free click chemistry capability of the azadibenzocyclooctyne (ADIBO) group with the strong affinity of biotin for streptavidin, enabling efficient two-step target isolation and analysis. This conjugate is essential for researchers and developers in pharmaceutical R&D, proteomics, and diagnostic assay development who require precise and efficient biomolecule tagging.

Application

• Bioorthogonal Labeling in Live Cells: For tagging azide-modified biomolecules (e.g., glycans, proteins) via strain-promoted alkyne-azide cycloaddition (SPAAC) without cytotoxic copper catalysts.
• Affinity Purification & Pull-Down Assays: Enables the isolation and enrichment of azide-tagged targets using streptavidin-coated beads or columns following the initial click reaction.
• Proteomic Profiling: Used in chemical proteomics to identify, quantify, and study post-translationally modified proteins or drug targets.
• Diagnostic Probe Development: Serves as a key component in developing sensitive detection kits and imaging probes for research and clinical diagnostics.
• Antibody-Drug Conjugate (ADC) Research: Facilitates site-specific conjugation and analysis in the development of novel biotherapeutics.
• Materials Science & Surface Functionalization: For creating biotinylated surfaces or materials with specific bio-recognition properties.

Basic Information

Item	Details
Product Name	Azadibenzocyclooctyne-Biotin Conjugate
CAS No.	1418217-95-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	ADIBO-Biotin; Biotin-ADIBO; Azadibenzocyclooctyne-PEGn-Biotin (variable linker); Biotinylated ADIBO; DBCO-Biotin analog; SPAAC-Biotin Conjugate; 3,5-Bis((1S,8S)-8-biotinyl-3,6-dioxaoctyl)-4-(4-((1S,8S)-8-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)-3,6-dioxaoctyl)phenyl)-1,2,4-triazole
EINECS	Contact for details

Quality Control

Every batch of our Azadibenzocyclooctyne-Biotin Conjugate undergoes rigorous analytical testing to ensure identity, purity, and functional performance. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as purity by HPLC, functional group integrity, and moisture content. Our quality management system supports compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.