Description

Purotoxin 1 is a highly selective peptide toxin isolated from the venom of the spider *Pamphobeteus antinous*, known for its potent and reversible inhibition of voltage-gated sodium channels (Nav1.7). It serves as a critical pharmacological tool for neuroscience research, pain pathway studies, and ion channel drug discovery due to its exceptional target specificity and low off-target activity. Researchers in academic institutions, biopharmaceutical R&D labs, and contract research organizations rely on Purotoxin 1 CAS NO 1396322-38-5 for mechanistic electrophysiology assays, high-throughput screening, and structure–activity relationship (SAR) profiling.

Application

• Electrophysiological characterization of Nav1.7 sodium channel function in heterologous expression systems (e.g., HEK293, CHO cells)
• Preclinical validation of Nav1.7-targeted analgesics in rodent models of neuropathic and inflammatory pain
• Reference standard in mass spectrometry (LC-MS/MS) and HPLC method development for peptide toxin quantification
• Structural biology studies including NMR and X-ray crystallography of toxin–channel complexes
• Development of antibody-based detection reagents and immunoassays for sodium channel modulation
• In vitro functional selectivity profiling against related voltage-gated ion channels (Nav1.1–Nav1.9, Cav, Kv)
• Tool compound for CRISPR/Cas9-edited neuronal cell lines to dissect Nav1.7-dependent signaling pathways
• Quality control reference material for biosynthetic or recombinant production of engineered toxin analogs

Basic Information

Product Name	Purotoxin 1
CAS No.	1396322-38-5
Molecular Formula	C149H235N43O39S6
Molecular Weight	3422.0 g/mol
Synonyms	Purotoxin-1; PTX-1; Pamphobeteus antinous toxin 1; PaTX-1; Nav1.7-selective inhibitory peptide; Spider venom peptide inhibitor; Sodium channel blocker peptide; Toxin Bm1a homolog
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch of Purotoxin 1 is validated for identity, purity (>95% by analytical RP-HPLC), endotoxin levels (<1.0 EU/mg), and biological activity (IC50 against human Nav1.7 confirmed via patch-clamp assay). Certificates of Analysis (COA) are available upon request, and manufacturing adheres to ISO 9001 and cGMP-aligned quality management practices.

Storage

Preserve in a tightly closed container, protected from light. Store at –20°C under inert atmosphere (argon or nitrogen) to prevent oxidation and degradation. Due to its hygroscopic nature, minimize exposure to ambient humidity; desiccated storage with silica gel is recommended. Allow vials to equilibrate to room temperature in a desiccator before opening to avoid condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time matches reference standard
Identification (Mass Spectrometry)	[M+3H]3+ m/z 1141.3 ± 0.5 (calcd. 1141.3)
Assay (HPLC)	≥95.0%
Related Substances	Single impurity ≤1.0%; Total impurities ≤3.0%
Water Content (KF)	≤8.0%
Residual Solvents	MeOH ≤3000 ppm; Acetonitrile ≤400 ppm
Endotoxin	<1.0 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.