Description

Histone H3 (21-44) CAS NO 1373516-71-2 is a defined peptide fragment corresponding to amino acid residues 21 to 44 of the human Histone H3 protein. This synthetic peptide is a critical research tool for studying post-translational modifications, protein-protein interactions, and epigenetic regulation mechanisms. It is essential for researchers and developers in the fields of epigenetics, oncology, and molecular biology, serving as a substrate, antigen, or standard in various biochemical assays.

Application

• Epigenetic Research: Used as a substrate for studying specific methylation, acetylation, phosphorylation, and other post-translational modifications on the Histone H3 tail.
• Antibody Production & Validation: Serves as an immunogen for generating or as a target for validating antibodies specific to the Histone H3 (21-44) epitope.
• Biochemical Assay Development: Employed as a critical component or standard in ELISA, western blot, pull-down assays, and other interaction studies.
• Chromatin & Nuclear Biology Studies: Utilized to investigate histone-modifying enzyme activity (e.g., histone methyltransferases, acetyltransferases) and chromatin remodeling complexes.
• Drug Discovery Screening: Acts as a target in high-throughput screens for identifying novel therapeutics that modulate histone-related pathways in diseases like cancer.
• Diagnostic Development: Potential use in developing assays to detect specific histone modifications linked to disease states.

Basic Information

Item	Detail
Product Name	Histone H3 (21-44)
CAS No.	1373516-71-2
Molecular Formula	C₁₁₇H₁₉₇N₄₅O₃₈
Molecular Weight	2635.9 g/mol (monoisotopic)
Synonyms	H3 (21-44) Peptide; Histone H3 Peptide Fragment 21-44; H3K Peptide Substrate; ARTKQTARKSTGGKAPRKQLASK; Acetyl-Histone H3 (21-44); Methyl-Histone H3 (21-44); Epigenetic Research Peptide; Chromatin Peptide
EINECS	Contact for details

Quality Control

Our Histone H3 (21-44) is manufactured under stringent conditions to ensure the highest standards of purity and batch-to-batch consistency for research applications. Each lot is accompanied by comprehensive analytical documentation. Certificates of Analysis (COA) are provided, detailing purity (typically >95% by HPLC), identity confirmation by mass spectrometry (MS), and other relevant physicochemical data.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70% (by weight)
Amino Acid Sequence	ARTKQTARKSTGGKAPRKQLASK
Solubility	Soluble in water or aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.