Description

Biotin-Peg6-T-Butyl Ester is a high-purity, heterobifunctional PEG linker featuring a biotin moiety for affinity-based applications and a protected carboxylic acid for controlled conjugation. This compound is essential for researchers and manufacturers requiring precise, stable, and site-specific biotinylation of biomolecules, peptides, or surfaces. It is primarily utilized in the development of diagnostic assays, targeted drug delivery systems, and advanced bioconjugation platforms within the pharmaceutical and life science industries.

Application

• Bioconjugation Reagent: For the site-specific labeling of proteins, antibodies, and peptides with biotin for downstream detection or purification.
• Diagnostic Assay Development: Used to create biotinylated probes and capture molecules in ELISA, lateral flow assays, and other immunoassay formats.
• Drug Delivery Systems: Serves as a key component in constructing ligand-targeted therapeutics, where the PEG spacer enhances solubility and reduces immunogenicity.
• Affinity Chromatography: Immobilization onto solid supports to create streptavidin/avidin-based purification resins for high-affinity capture.
• Surface Functionalization: Modification of nanoparticles, biosensors, and microarray slides to introduce biotin groups for subsequent binding studies.
• Chemical Biology & Proteomics: A versatile tool for pull-down assays, protein interaction studies, and activity-based protein profiling.

Basic Information

Product Name	Biotin-Peg6-T-Butyl Ester
CAS No.	1352814-07-3
Molecular Formula	C35H64N4O11S
Molecular Weight	748.97 g/mol
Synonyms	Biotin-PEG6-t-butyl ester; Biotin-PEG6-OtBu; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-[2-[2-[2-(1,1-dimethylethoxy)-2-oxoethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethyl]pentanamide; Biotinamidohexa(ethylene glycol) t-butyl ester; Biotin-PEG6-COOtBu; Biotinylated PEG6 t-butyl ester; Biotin-(PEG)6-t-butyl ester
EINECS	Contact for details

Quality Control

Our Biotin-Peg6-T-Butyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high chemical purity and structural integrity. Certificates of Analysis (COA) detailing identity, purity, and impurity profiles are provided with every shipment to support your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.