Description

Biotinyl Cystamine Hydrochloride CAS NO 1346597-28-1 is a high-purity biochemical reagent featuring a biotin tag linked via a cleavable disulfide bond. This compound is critical for applications requiring reversible biotinylation, enabling the capture and subsequent release of target molecules under mild reducing conditions. It is an essential building block for researchers and manufacturers in the fields of proteomics, affinity purification, and diagnostic assay development.

Application

• Affinity Chromatography & Protein Purification: Used to create reversible biotin-streptavidin complexes for the isolation and gentle elution of proteins, peptides, and other biomolecules.
• Proteomics & Biomarker Discovery: Serves as a key reagent for labeling and capturing target proteins from complex biological samples for mass spectrometry analysis.
• Diagnostic Kit Manufacturing: Employed in the development of ELISA and lateral flow assays where controlled release of captured analytes is required.
• Drug Delivery & Bioconjugation: Facilitates the creation of cleavable drug-linker systems in antibody-drug conjugates (ADCs) and targeted therapeutics.
• Surface Plasmon Resonance (SPR): Used to functionalize sensor chips with biotin for capturing ligands that can be regenerated under reducing conditions.
• Cell Surface Labeling & Tracking: Enables reversible tagging of cell surface proteins for isolation and subsequent study.

Basic Information

Product Name	Biotinyl Cystamine Hydrochloride
CAS No.	1346597-28-1
Molecular Formula	C14H26N4O3S2 • HCl
Molecular Weight	422.98 g/mol
Synonyms	Biotin-Cystamine•HCl; Biotinylated Cystamine Hydrochloride; N-Biotinylcystamine Hydrochloride; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-[2-(2-aminoethyl)disulfanyl]ethyl]pentanamide Hydrochloride; Biotin SS Amine; Biotin Disulfide Amine; Biotin-HPDP (reduced form precursor); Cleavable Biotinylation Reagent
EINECS	Contact for details

Quality Control

Our Biotinyl Cystamine Hydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals (as Pb)	≤ 10 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.