Description

Fmoc-n-Amido-(Peg4-Biotin)-Acid CAS NO 1334172-63-2 is a sophisticated, heterobifunctional linker molecule designed for advanced bioconjugation and proteomics research. This compound integrates an amine-reactive Fmoc-protected amino group, a long, flexible tetraethylene glycol (PEG4) spacer, and a biotin moiety for high-affinity streptavidin/avidin capture. It is engineered to enable the controlled synthesis and purification of peptide and protein conjugates, serving as a critical tool for researchers in drug discovery and diagnostic development. This high-purity biochemical is essential for laboratories focused on creating targeted biotherapeutics, developing novel assay platforms, and exploring complex biomolecular interactions.

Application

• Solid-Phase Peptide Synthesis (SPPS): Used as a specialized building block for the site-specific incorporation of biotin tags during Fmoc-based peptide synthesis.
• Affinity Probe Development: Enables the creation of biotinylated probes for pull-down assays, target identification, and protein-protein interaction studies.
• ADC (Antibody-Drug Conjugate) Linker Exploration: Serves as a model or functional component in the design of novel linkers, leveraging its PEG spacer for improved solubility and pharmacokinetics.
• Diagnostic Reagent Manufacturing: A key intermediate in the production of biotin-labeled antibodies, antigens, and oligonucleotides for ELISA, lateral flow assays, and other diagnostic kits.
• Surface Functionalization: Facilitates the biotinylation of chips, beads, or other surfaces for biosensor development and microarray technology.
• Chemical Biology Toolkits: Provides a versatile handle for labeling, immobilizing, or detecting biomolecules in complex biological systems.

Basic Information

Item	Detail
Product Name	Fmoc-n-Amido-(Peg4-Biotin)-Acid
CAS No.	1334172-63-2
Molecular Formula	C₄₀H₅₆N₄O₁₀S
Molecular Weight	784.97 g/mol
Synonyms	Fmoc-N-amido-PEG4-biotin; 5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-[[(9H-fluoren-9-ylmethoxy)carbonyl]amino]ethoxy]ethoxy]ethoxy]ethyl]pentanamide; Fmoc-Peg4-Biotin; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-1-amino-3,6,9,12-tetraoxapentadecan-15-oic acid, 15-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl] ester; Biotin-PEG4-Fmoc; Fmoc-AEEA-AEEA-AEEA-AEEA-Biotin
EINECS	Contact for details

Quality Control

Every batch of Fmoc-n-Amido-(Peg4-Biotin)-Acid is manufactured under strict quality management systems and undergoes comprehensive analytical characterization to ensure identity, purity, and consistency. We provide full traceability and Certificates of Analysis (COA) detailing results from HPLC, NMR, and mass spectrometry. Our production adheres to cGMP guidelines where applicable, supporting its use in research and development phases of pharmaceutical and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.