Description

Fmoc-Asp(Otbu)-Oh (U-13C4, 15N) is a high-purity, isotopically labeled amino acid derivative essential for advanced research and development. This compound is specifically designed for use as a stable isotope-labeled building block in peptide synthesis, enabling precise metabolic and pharmacokinetic studies. It is a critical material for pharmaceutical researchers, analytical chemists, and biotech companies engaged in proteomics, tracer studies, and the development of novel peptide-based therapeutics.

Application

• Stable Isotope Labeled Peptide Synthesis: Primary building block for the solid-phase synthesis of peptides containing uniformly labeled aspartic acid for mass spectrometry-based quantification.
• Metabolic Pathway Tracing: Used in 13C and 15N tracer studies to investigate protein metabolism, amino acid flux, and biochemical pathways in vivo and in vitro.
• Quantitative Proteomics & Biomarker Discovery: Enables the accurate quantification of proteins and peptides in complex biological samples using techniques like SILAC (Stable Isotope Labeling by Amino acids in Cell culture) or AQUA.
• Pharmaceutical R&D: Critical for the development and analysis of peptide drugs, allowing for detailed pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies.
• NMR Spectroscopy Studies: The 13C and 15N labels provide distinct signals for structural elucidation and dynamics studies of peptides and proteins using nuclear magnetic resonance.
• Reference Standard Production: Serves as a certified reference material for the calibration of analytical instruments and validation of methods in regulatory environments.

Basic Information

Product Name	Fmoc-Asp(Otbu)-Oh (U-13C4, 15N)
CAS No.	1217468-27-3
Molecular Formula	C23H2213C415NO6
Molecular Weight	~445.4 g/mol (for labeled form)
Synonyms	Fmoc-L-aspartic acid 4-tert-butyl ester (U-13C4, 15N); N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-aspartic acid 1-tert-butyl ester (U-13C4, 15N); Fmoc-Asp(OtBu)-OH (U-13C4, 15N); Fmoc-L-Asp(OtBu)-OH (U-13C4, 15N); (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonylamino]butanedioic acid 4-tert-butyl ester (U-13C4, 15N); Fmoc-Aspartic acid tert-butyl ester (U-13C4, 15N); Stable Isotope Labeled Fmoc-Asp(OtBu)-OH
EINECS	Contact for details

Quality Control

Every batch of Fmoc-Asp(Otbu)-Oh (U-13C4, 15N) is manufactured and analyzed under strict quality systems to ensure the highest isotopic and chemical purity for sensitive research applications. Our products undergo rigorous quality testing, including advanced spectroscopic and chromatographic methods, to ensure compliance with exacting customer specifications. Comprehensive Certificates of Analysis (COA) are provided, detailing isotopic enrichment (Atom % 13C and 15N), chemical purity, and identity confirmation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This material is hygroscopic (moisture-sensitive) and must be kept under dry, inert conditions. Allow the sealed container to reach room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (13C)	≥ 99 atom % 13C
Isotopic Enrichment (15N)	≥ 98 atom % 15N
Optical Rotation	Specific range provided on COA
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.