Description

n-Fmoc-(15N)-L-Tyrosine o-Tertbutyl Ether is a specialized, isotopically labeled amino acid derivative designed for advanced research and development. This compound is a critical building block in solid-phase peptide synthesis (SPPS), where the Fmoc-protected amine and the tert-butyl protected phenolic hydroxyl group provide orthogonal protection for precise molecular assembly. It is primarily utilized by pharmaceutical researchers, biochemists, and CROs engaged in the synthesis of isotopically labeled peptides for applications in mass spectrometry, metabolic studies, and structural biology. The incorporation of the 15N isotope offers a non-radioactive tracer for detailed mechanistic and analytical investigations.

Application

• Isotope-Labeled Peptide Synthesis: A key precursor for incorporating 15N-labeled tyrosine residues into custom peptides for Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry-based proteomics.
• Pharmaceutical R&D: Used in the research and development of novel peptide-based therapeutics, enabling pharmacokinetic and metabolic pathway tracing studies.
• Biomarker Discovery: Essential for creating internal standards in quantitative proteomics and metabolomics to accurately measure protein expression and metabolite concentrations.
• Structural Biology: Facilitates the production of labeled proteins for advanced NMR structural analysis and dynamics studies.
• Academic Research: Supports fundamental research in biochemistry, enzymology, and molecular biology involving tyrosine phosphorylation sites and signal transduction pathways.
• Contract Research & Manufacturing (CRAM): A vital raw material for CROs and CDMOs offering custom peptide synthesis services with isotopic labeling capabilities.

Basic Information

Product Name	n-Fmoc-(15N)-L-Tyrosine o-Tertbutyl Ether
CAS No.	1217437-39-2
Molecular Formula	C28H2915NO5
Molecular Weight	~463.5 g/mol (for 15N isotopologue)
Synonyms	Fmoc-Tyr(tBu)-OH-15N
N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-15N-L-tyrosine O-tert-butyl ether
(2S)-2-[[(9H-Fluoren-9-ylmethoxy)carbonyl]-15N]amino-3-(4-tert-butoxyphenyl)propanoic acid
Fmoc-15N-L-Tyrosine(O-tBu)-OH
15N-Labeled Fmoc-Tyr(O-tBu)-OH
N-α-Fmoc-15N-L-tyrosine O-tert-butyl ester (common misnomer for the ether)
Contact for details on EINECS or other regulatory identifiers.

Quality Control

This high-purity, isotopically labeled building block is manufactured under strict quality control protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment, chemical purity, and chiral integrity. Our quality system is designed to meet the stringent requirements of GMP and ISO guidelines for research chemicals, ensuring traceability and compliance for global pharmaceutical and academic customers.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term storage, consider under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
15N Isotopic Enrichment	≥ 98 atom % 15N
Specific Rotation	[α]D20: Contact for details
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.