Description

Fmoc-(7-Hydroxycoumarin-4-Yl)-Ethyl-Gly-Oh, Fmoc-(Umbellifer-4-Yl)-Ethyl-Gly-Oh is a specialized, fluorogenic amino acid derivative designed for advanced peptide synthesis and biochemical research. This compound matters because it incorporates the highly sensitive 7-hydroxycoumarin (umbelliferone) fluorophore, protected by an Fmoc group, enabling the synthesis of peptides with built-in fluorescence for real-time detection and analysis. It is essential for researchers and manufacturers in pharmaceutical R&D, proteomics, and diagnostic assay development who require precise, traceable labeling and monitoring of peptide interactions and enzymatic activity.

Application

• Solid-Phase Peptide Synthesis (SPPS): As a protected, fluorescently-tagged amino acid building block for the Fmoc/t-Bu strategy.
• Fluorescent Peptide & Protein Labeling: For creating custom peptide probes with intrinsic fluorescence for bioimaging and cellular tracking.
• Protease & Enzyme Activity Assays: Serving as a key substrate or quenched fluorescent reporter (FRET pairs) to monitor enzymatic cleavage kinetics.
• Bioconjugation & Chemical Biology: Enabling the site-specific introduction of a fluorescent tag into biomolecules for interaction studies.
• Diagnostic Reagent Development: Used in the formulation of sensitive detection kits for clinical diagnostics and high-throughput screening.
• Academic & Industrial Research: A critical tool in studying protein-protein interactions, signal transduction pathways, and drug discovery mechanisms.

Basic Information

Product Name	Fmoc-(7-Hydroxycoumarin-4-Yl)-Ethyl-Gly-Oh, Fmoc-(Umbellifer-4-Yl)-Ethyl-Gly-Oh
CAS No.	1187744-84-8
Molecular Formula	C₂₉H₂₅NO₇
Molecular Weight	499.51 g/mol
Synonyms	Fmoc-Aoa(7-hydroxycoumarin-4-yl)-OH; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-3-[2-(7-hydroxy-2-oxo-2H-chromen-4-yl)ethylamino]-2-(9H-fluoren-9-ylmethoxycarbonylamino)propanoic acid; Fmoc-3-[2-(7-hydroxy-2-oxo-2H-1-benzopyran-4-yl)ethylamino]-2-(9H-fluoren-9-ylmethoxycarbonylamino)propanoic acid; Fmoc-Lys(7-hydroxycoumarin-4-yl-ethyl)-OH; Fluorenylmethyloxycarbonyl-(7-hydroxycoumarin-4-yl)ethylglycine; Fmoc-K(7-hydroxycoumarin-4-yl-ethyl)-OH; Fmoc-(Coumarin-4-Yl)-Ethyl-Gly-Oh
EINECS	Contact for details

Quality Control

Our Fmoc-(7-Hydroxycoumarin-4-Yl)-Ethyl-Gly-Oh is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research applications. All batches undergo comprehensive analytical characterization including HPLC, NMR, and mass spectrometry to confirm identity and purity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting critical specifications such as assay, chiral purity, and impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Chiral Purity	≥ 99.0% ee
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.