Description

(S)-2-((((9H-Fluoren-9-Yl)Methoxy)Carbonyl)(Methyl)Amino)-3,3-Dimethylbutanoic Acid is a high-purity, protected amino acid derivative, specifically an Fmoc-protected tert-leucine analog with a methylated amide nitrogen. This compound is a critical building block in solid-phase peptide synthesis (SPPS), enabling the controlled introduction of sterically hindered, non-proteinogenic amino acids to modulate peptide stability and bioactivity. It is essential for researchers and manufacturers in pharmaceutical development, particularly for creating novel therapeutic peptides, peptidomimetics, and bioactive probes. The product, CAS NO 1172579-62-2, is supplied to meet the stringent demands of advanced R&D and cGMP manufacturing.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key intermediate for the automated, sequential assembly of custom peptide sequences, especially those requiring the incorporation of (S)-tert-leucine derivatives.
• Pharmaceutical R&D: Used in the discovery and development of novel peptide-based therapeutics, including hormone analogs, enzyme inhibitors, and targeted drug conjugates.
• Peptidomimetic Design: Facilitates the creation of metabolically stable peptide mimics by introducing the bulky, hydrophobic tert-butyl side chain from tert-leucine.
• Bioconjugation & Probe Synthesis: Serves as a specialized linker or modified amino acid component in the synthesis of fluorescent probes, affinity tags, and peptide-drug conjugates.
• Academic Research: Employed in biochemistry and chemical biology studies to investigate protein-protein interactions, enzyme specificity, and structure-activity relationships (SAR).
• Process Development: Used for scaling up the production of peptide active pharmaceutical ingredients (APIs) under controlled GMP environments.

Basic Information

Product Name	(S)-2-((((9H-Fluoren-9-Yl)Methoxy)Carbonyl)(Methyl)Amino)-3,3-Dimethylbutanoic Acid
CAS No.	1172579-62-2
Molecular Formula	C22H25NO5
Molecular Weight	383.44 g/mol
Synonyms	Fmoc-N-Me-tert-leucine-OH; Fmoc-N-Methyl-tert-leucine; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-N-methyl-L-tert-leucine; (S)-2-((((9H-Fluoren-9-yl)methoxy)carbonyl)(methyl)amino)-3,3-dimethylbutanoic acid; Fmoc-N-Me-Tle-OH; Nα-Fmoc-Nα-methyl-L-tert-leucine; N-Fmoc-N-methyl-L-tert-leucine; Nα-(9-Fluorenylmethoxycarbonyl)-Nα-methyl-L-tert-leucine
EINECS	Contact for details

Quality Control

Our (S)-2-((((9H-Fluoren-9-Yl)Methoxy)Carbonyl)(Methyl)Amino)-3,3-Dimethylbutanoic Acid is manufactured under strict quality systems. Every batch undergoes comprehensive analytical testing, including HPLC for purity and chiral integrity, NMR for structural confirmation, and mass spectrometry to ensure identity and meet specifications suitable for peptide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided for traceability and regulatory support.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This material is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere after each use to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Chiral Purity (HPLC)	≥99.0%
Water Content (KF)	≤0.5%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.