Description

Gap 27 is a synthetic, cell-permeable peptide known for its potent and selective inhibition of connexin 43 (Cx43) hemichannels. This compound is a critical research tool for investigating the role of gap junction communication and hemichannel activity in cellular physiology and disease pathology. It is primarily utilized by researchers in academic, pharmaceutical, and biotechnology laboratories focused on cardiology, neuroscience, oncology, and wound healing.

Application

• Cardiovascular Research: Used to study ischemia/reperfusion injury, cardiac arrhythmias, and myocardial infarction by blocking Cx43 hemichannels in cardiomyocytes.
• Neuroscience Studies: Applied in models of stroke, epilepsy, and neuroinflammation to understand the role of astrocytic hemichannels in neuronal death and glial signaling.
• Oncology Research: Investigated for its effects on cancer cell proliferation, migration, and the tumor microenvironment by modulating intercellular communication.
• Wound Healing & Dermatology: Explored in studies of skin repair, inflammation, and conditions like psoriasis by affecting keratinocyte and fibroblast communication.
• Inflammation Models: Utilized to probe the release of ATP and glutamate through hemichannels in various inflammatory disease models.
• Basic Cell Biology: Serves as a standard pharmacological tool to dissect the specific functions of Cx43 versus other connexin isoforms in vitro and in vivo.

Basic Information

Product Name	Gap 27
CAS No.	198284-64-9
Molecular Formula	C78H134N26O27S2
Molecular Weight	1844.2 g/mol
Synonyms	Gap27; Connexin 43 Inhibitor, Gap 27; Cx43 Inhibitor Peptide; SRPTEKTIFII; Peptide Gap 27; H-Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile-NH2; Acetyl-SRPTEKTIFII-NH2; Connexin-mimetic Peptide Gap27
EINECS	Contact for details

Quality Control

Our Gap 27 is manufactured under strict quality protocols suitable for research applications. Each batch undergoes comprehensive analytical testing including HPLC for purity verification and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing purity, peptide content, and solvent residues are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the lyophilized powder at -20°C or below. Once reconstituted in a suitable buffer (e.g., sterile water or PBS), aliquot and store at -20°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Single Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%
Acetate Content (HPLC)	≤ 10.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.