Description

H-Leu-Val-Leu-Ala-Pna is a synthetic peptide substrate designed for biochemical research and pharmaceutical development. This compound is a critical tool for studying protease enzyme activity, kinetics, and inhibition, providing valuable insights into biological pathways. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical R&D, proteomics, and diagnostic assay development.

Application

• Protease Enzyme Substrate: Used as a specific chromogenic or fluorogenic substrate for studying the activity and specificity of various proteases, including caspases and other serine or cysteine proteases.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) assays to identify and characterize potential protease inhibitor candidates for therapeutic development.
• Biochemical Research: Essential for fundamental research into apoptosis (programmed cell death), inflammation, and other cellular processes regulated by protease activity.
• Diagnostic Kit Development: Integrated into the development of in-vitro diagnostic (IVD) kits and reagents for detecting specific enzyme activities related to disease states.
• Enzyme Kinetics Studies: Facilitates the determination of kinetic parameters (Km, Vmax, kcat) for target enzymes, aiding in mechanistic studies.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity of enzyme-based products or processes within biopharmaceutical manufacturing.

Basic Information

Item	Detail
Product Name	H-Leu-Val-Leu-Ala-Pna
CAS No.	197970-37-9
Molecular Formula	C27H42N6O6
Molecular Weight	546.66 g/mol
Synonyms	H-Leu-Val-Leu-Ala-pNA; L-Leucyl-L-valyl-L-leucyl-L-alanine 4-nitroanilide; Ac-Leu-Val-Leu-Ala-pNA; Leu-Val-Leu-Ala-pNA; Caspase-3 Substrate I (Ac-DEVD-pNA analog); Peptide substrate pNA; Protease substrate 197970-37-9; L-Leu-L-Val-L-Leu-L-Ala-4-nitroanilide
EINECS	Contact for details

Quality Control

Our H-Leu-Val-Leu-Ala-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, mass spectrometry (MS) for identity confirmation, and NMR for structural validation. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC & MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.