Description

Sulpho Nhs Biotin CAS NO 194041-65-1 is a high-purity, activated ester derivative of biotin, specifically designed for bioconjugation applications. This compound is critical for researchers and manufacturers requiring efficient, site-specific labeling of biomolecules, such as proteins and antibodies, with biotin. It is an essential reagent for the life sciences, pharmaceutical, and diagnostic industries, enabling advanced techniques in affinity purification, immunoassays, and cellular imaging.

Application

• Bioconjugation Reagent: Primary use for covalently attaching biotin to primary amines (-NH2) on proteins, peptides, and antibodies.
• Affinity Chromatography Probe Synthesis: Creation of biotinylated ligands for capture on streptavidin/avidin columns.
• Diagnostic Assay Development: Labeling of detection antibodies in ELISA, lateral flow assays, and other immunoassay platforms.
• Cellular Imaging and Flow Cytometry: Preparation of biotinylated probes for visualizing cellular targets via fluorescence-activated cell sorting (FACS) or microscopy.
• Drug Delivery & Targeting: Functionalization of nanoparticles or therapeutic agents for targeted delivery systems.
• Protein Interaction Studies: Tagging proteins for pull-down assays to study protein-protein interactions.
• Microarray and Biosensor Fabrication: Immobilization of biomolecules on sensor surfaces for high-throughput screening.

Basic Information

Product Name	Sulpho Nhs Biotin
CAS No.	194041-65-1
Molecular Formula	C20H30N4O9S2
Molecular Weight	534.60 g/mol
Synonyms	Sulfo-NHS-Biotin; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt; (+)-Biotin-6-amidocaproic acid 3-sulfo-N-hydroxysuccinimide ester; Sulfo-NHS-LC-Biotin; EZ-Link Sulfo-NHS-LC-Biotin; N-ε-Biotinyl-6-aminocaproic acid N-hydroxysulfosuccinimide ester; 3-Sulfo-N-hydroxysuccinimide biocytinamide; Biotinylation reagent, sulfo-NHS ester
EINECS	Contact for details

Quality Control

Our Sulpho Nhs Biotin is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is tested to meet stringent in-house specifications, with comprehensive analysis including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.