Description

Fmoc-Glu(Edans)-Otbu is a specialized, high-purity protected amino acid derivative designed for advanced peptide synthesis and biochemical research. This compound is critical for researchers and manufacturers requiring precise, fluorescently labeled building blocks for solid-phase peptide synthesis (SPPS), particularly in the development of FRET-based substrates and probes. It is primarily utilized by pharmaceutical R&D teams, biotechnology companies, and academic research institutions focused on protease activity assays, enzyme kinetics studies, and the development of novel diagnostic tools.

Application

• FRET Substrate Synthesis: A key building block for creating fluorescence resonance energy transfer (FRET) peptide substrates used to study protease activity and enzyme kinetics.
• Protease Research & Assay Development: Essential for developing sensitive and specific assays to detect and quantify protease enzymes like caspases, MMPs, and thrombin.
• Solid-Phase Peptide Synthesis (SPPS): Used as a fluorescently labeled, Fmoc-protected glutamic acid derivative for the automated synthesis of custom peptides.
• Biochemical Probe Design: Incorporated into peptides to create molecular probes for real-time monitoring of biochemical processes in cell-based and in vitro studies.
• Drug Discovery Platforms: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic compounds that modulate protease function.
• Diagnostic Reagent Manufacturing: Serves as a critical raw material in the production of diagnostic kits for diseases where protease biomarkers are relevant.

Basic Information

Product Name	Fmoc-Glu(Edans)-Otbu
CAS No.	194039-49-1
Molecular Formula	C₃₃H₃₅N₃O₉S
Molecular Weight	625.71 g/mol
Synonyms	Fmoc-Glu(EDANS)-OtBu; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-5-[(5-dimethylamino-1-naphthalenesulfonyl)amino]-L-glutamic acid 1-tert-butyl ester; Fmoc-L-glutamic acid 5-(5-dimethylaminonaphthalene-1-sulfonamide), 1-tert-butyl ester; Fmoc-Glu(Edans)-O-tert-butyl; EDANS-labeled Fmoc-Glutamic acid tert-butyl ester; (2S)-2-[(9H-Fluoren-9-ylmethoxy)carbonylamino]-5-[[5-(dimethylamino)naphthalene-1-sulfonyl]amino]-5-oxopentanoic acid tert-butyl ester
EINECS	Contact for details

Quality Control

Our Fmoc-Glu(Edans)-Otbu is manufactured under strict quality control protocols to ensure the high purity and consistency required for sensitive research and diagnostic applications. Each batch is analyzed using advanced techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, it is recommended to store under an inert atmosphere. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.