Description

H-His-Amc is a specialized fluorogenic peptide substrate designed for the sensitive detection and quantification of specific enzymatic activity. This compound is of significant value in biochemical research and diagnostic development due to its role in generating a measurable fluorescent signal upon cleavage. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for applications in enzyme kinetics, high-throughput screening, and assay development.

Application

• Enzyme Activity Assays: Used as a specific substrate for proteases and other hydrolytic enzymes, particularly in kinetic studies.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify potential enzyme inhibitors or activators.
• Diagnostic Kit Development: Serves as a key component in the development of fluorometric diagnostic tests for clinical and research use.
• Biochemical Research: Utilized to study enzyme specificity, mechanism of action, and catalytic efficiency in vitro.
• Cell-Based Assays: Applied in live-cell imaging and cellular activity studies where fluorogenic substrates are required.
• Quality Control in Biologics: Acts as a tool for monitoring enzyme activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	H-His-Amc
CAS No.	191723-64-5
Molecular Formula	C17H21N5O4
Molecular Weight	359.38 g/mol
Synonyms	L-Histidyl-7-amido-4-methylcoumarin; Histidine-AMC; H-His-AMC; His-AMC; 7-Amido-4-methylcoumarin conjugate of L-Histidine; Fluorogenic substrate H-His-AMC; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-histidyl-7-amido-4-methylcoumarin (Fmoc variant); Substrate for histidine-specific proteases
EINECS	Contact for details

Quality Control

Our H-His-Amc is produced under stringent quality management protocols to ensure batch-to-batch consistency and high purity. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.