Description

(Phe22)-Big Endothelin-1 Fragment (19-37) (Human) is a synthetic peptide fragment derived from the human Big Endothelin-1 precursor, specifically modified with a phenylalanine substitution at position 22. This compound is a critical research tool for investigating the structure-activity relationships, processing, and receptor interactions of endothelin peptides, which are potent vasoconstrictors. It is primarily utilized by researchers and developers in the fields of cardiovascular pharmacology, peptide biochemistry, and drug discovery.

Application

• Biochemical Research: Used as a reference standard and substrate in studies of endothelin-converting enzyme (ECE) activity and specificity.
• Pharmacological Studies: Essential for investigating the structure-activity relationship (SAR) of Big Endothelin-1 and its conversion to active endothelin isoforms.
• Drug Discovery: Serves as a key intermediate or target molecule in the development of novel cardiovascular therapeutics and enzyme inhibitors.
• Antibody Production: Can be used as an immunogen or antigen for generating specific antibodies against Big Endothelin-1 fragments.
• Cell Signaling Research: Applied in in vitro assays to study endothelin receptor binding and downstream signaling pathways.
• Quality Control: Functions as a high-purity standard for the analysis and validation of related peptide products in manufacturing.

Basic Information

Product Name	(Phe22)-Big Endothelin-1 Fragment (19-37) (Human)
CAS No.	189064-07-1
Molecular Formula	C106H158N28O30S2
Molecular Weight	2352.7 g/mol
Synonyms	Big ET-1 (19-37), Phe(22)-substituted; [Phe22]-Big Endothelin-1 (19-37); Human Big Endothelin-1 Fragment 19-37 (Phe22); ET-1 Precursor Fragment (19-37); Big Endothelin-1 (19-37) Peptide; Big ET (19-37); Endothelin-1 Big Fragment; H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-OH (Disulfide bridge: Cys1-Cys15)
EINECS	Contact for details

Quality Control

Our (Phe22)-Big Endothelin-1 Fragment (19-37) (Human) is produced under stringent conditions to ensure the highest standards of purity and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including HPLC for purity, MS for identity confirmation, and amino acid analysis. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all specifications and test results to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated. Allow the vial to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with theoretical molecular weight
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80%
Amino Acid Analysis	Within ± 10% of theoretical composition
Water Content (KF)	≤ 5.0%
Acetate Content (HPLC)	≤ 10.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.