Description

5-Carboxyfluorescein Dipivalate n-Hydroxysuccinimide Ester is a highly reactive, amine-reactive derivative of the classic fluorescent dye, carboxyfluorescein. This compound is specifically engineered for the efficient, covalent labeling of proteins, peptides, antibodies, and other biomolecules containing primary amine groups, enabling the creation of bright, stable fluorescent conjugates. It is an essential tool for researchers and developers in bioconjugation, flow cytometry, immunoassay development, and fluorescence microscopy. The CAS number for this product is 186032-65-5.

Application

• Bioconjugation and Probe Synthesis: Primary application for labeling antibodies, proteins, peptides, and oligonucleotides with a bright, pH-insensitive fluorescent tag.
• Flow Cytometry: Creation of fluorescent antibody conjugates for cell surface marker detection and intracellular staining protocols.
• Immunoassay Development: Used in ELISA, Western blotting, and other immunoassays to generate fluorescent detection reagents.
• Fluorescence Microscopy & Imaging: Labeling of cellular structures or biomolecules for visualization in fixed and live-cell imaging.
• Diagnostic Kit Manufacturing: Key raw material in the production of fluorescent diagnostic reagents and kits.
• Drug Delivery & Targeting Research: Conjugation to nanoparticles, polymers, or targeting ligands to track distribution and uptake.

Basic Information

Product Name	5-Carboxyfluorescein Dipivalate n-Hydroxysuccinimide Ester
CAS No.	186032-65-5
Molecular Formula	C37H35NO11
Molecular Weight	669.68 g/mol
Synonyms	5-Carboxyfluorescein Diacetate NHS Ester; 5-CFDA SE; 6-Carboxyfluorescein Diacetate Succinimidyl Ester; 5(6)-Carboxyfluorescein Diacetate N-Succinimidyl Ester; CFDA, SE; CFDA-SE; 5(6)-CFDA, SE; CellTrace™ CFSE (related compound)
EINECS	Contact for details

Quality Control

We supply high-purity, research-grade material suitable for sensitive bioconjugation applications. Every batch is characterized to ensure performance and consistency. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing purity, identity, and performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%
Functional Performance	Conforms to labeling efficiency test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.