Description

Fmoc-Asp(Edans)-Otbu is a specialized, fluorescently-labeled amino acid derivative designed for advanced peptide synthesis and biochemical research. This compound is critical for researchers and manufacturers requiring high-purity, sequence-specific building blocks for fluorescence resonance energy transfer (FRET) studies and protease activity assays. It is primarily utilized by pharmaceutical R&D teams, biotechnology companies, and academic laboratories focused on developing diagnostic tools, enzyme substrates, and novel therapeutic peptides.

Application

• FRET-Based Peptide Substrates: Serves as a key component in the synthesis of fluorescent peptide substrates for monitoring protease activity (e.g., caspases, HIV protease) in real-time.
• Protease Assay Development: Used in high-throughput screening (HTS) platforms to discover and characterize new protease inhibitors for drug development.
• Solid-Phase Peptide Synthesis (SPPS): Acts as a protected, Fmoc-based building block for the automated synthesis of custom peptides containing the Edans fluorophore.
• Biochemical Research: Enables the study of enzyme kinetics, protein-protein interactions, and intracellular signaling pathways through fluorescence-based methodologies.
• Diagnostic Reagent Manufacturing: Incorporated into the production of sensitive diagnostic kits for clinical and research use, detecting specific enzymatic biomarkers.

Basic Information

Product Name	Fmoc-Asp(Edans)-Otbu
CAS No.	182253-74-3
Molecular Formula	C₃₄H₃₅N₃O₉S
Molecular Weight	637.72 g/mol
Synonyms	Fmoc-L-aspartic acid β-(Edans) tert-butyl ester; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-aspartic acid 4-[[5-[(dimethylamino)naphthalen-1-yl]sulfonyl]amino] tert-butyl ester; Fmoc-Asp(Edans)-O-t-butyl; Fmoc-L-Asp(Edans)-OtBu; 5-((2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)-4-(tert-butoxy)-4-oxobutanoyl)amino)naphthalene-1-sulfonic acid; N-α-Fmoc-N-β-(5-((2-aminoethyl)amino)naphthalene-1-sulfonyl)-L-aspartic acid tert-butyl ester (common misnomer); Edans-labeled Fmoc-Aspartate tert-butyl ester
EINECS	Contact for details

Quality Control

Our Fmoc-Asp(Edans)-Otbu is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and development. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via NMR and Mass Spectrometry. Certificates of Analysis (COA) detailing purity, chiral integrity, and impurity profiles are provided to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Chiral Purity	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.