Description

Dabcyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-Edans is a high-purity, synthetic peptide substrate designed for sensitive protease activity assays, particularly for HIV-1 protease. Its core value lies in the FRET (Fluorescence Resonance Energy Transfer) pair formed by the Dabcyl quencher and the Edans fluorophore, enabling precise, real-time measurement of enzymatic cleavage. This compound is essential for researchers and quality control laboratories in pharmaceutical development, biotechnology, and academic research requiring reliable, high-throughput screening tools. The product is supplied with guaranteed lot-to-lot consistency to ensure reproducible experimental results.

Application

• HIV-1 Protease Activity Assays: Primary substrate for kinetic studies, inhibitor screening, and enzymatic characterization of HIV-1 protease.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and evaluate potential antiviral compounds targeting HIV protease.
• Biochemical Research: A critical tool for studying protease mechanism, specificity, and function in virology and molecular biology.
• Diagnostic Kit Development: Serves as a key component in the development of fluorogenic assay kits for protease detection and quantification.
• Enzyme Kinetics: Enables the determination of kinetic parameters (Km, Vmax, kcat) for HIV-1 protease under various conditions.
• Quality Control in Pharma: Used for the potency testing and validation of HIV protease inhibitor drugs during manufacturing.

Basic Information

Product Name	Dabcyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-Edans
CAS No.	163265-38-1
Molecular Formula	C78H124N26O23S2
Molecular Weight	1786.1 g/mol
Synonyms	Dabcyl-GABA-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-Glu(Edans); HIV-1 Protease Substrate (Dabcyl/Edans); 5-((2-Aminoethyl)amino)naphthalene-1-sulfonyl-GABA-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-4-((4-(Dimethylamino)phenyl)azo)benzoic Acid; FRET HIV Protease Substrate; Dabcyl-GABA-RGVVNASSLRLAE-Edans; 5-((2-Aminoethyl)amino)naphthalene-1-sulfonyl-γ-aminobutyryl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-Glu(Edans); HIV-1 PR Substrate
EINECS	Contact for details

Quality Control

Every batch of Dabcyl-Arg-Gly-Val-Val-Asn-Ala-Ser-Ser-Arg-Leu-Ala-Edans is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and functional performance. Our quality system ensures compliance with relevant standards for research-grade biochemicals. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing results for HPLC purity, mass spectrometry (MS) confirmation, and functional activity in a standardized assay.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Functional Activity	Cleavage by HIV-1 protease produces expected increase in fluorescence
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.