Description

D-Val-Leu-Arg p-Nitroanilide Hydrochloride Salt is a high-purity, chromogenic peptide substrate designed for precise enzymatic activity detection. This compound is critical for biochemical research and diagnostic applications where accurate measurement of protease activity is required. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on enzymology and drug discovery.

Application

• Protease Activity Assays: As a specific chromogenic substrate for serine proteases, including thrombin, trypsin, and related enzymes.
• Diagnostic Reagent Manufacturing: A key component in the production of in-vitro diagnostic (IVD) kits for coagulation studies and other clinical tests.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: Employed in enzymology studies to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki) of target proteases.
• Quality Control in Biologics: Monitoring the activity and stability of enzyme-based biopharmaceutical products during manufacturing.

Basic Information

Product Name	D-Val-Leu-Arg p-Nitroanilide Hydrochloride Salt
CAS No.	162303-66-4
Molecular Formula	C24H39N9O6 • HCl
Molecular Weight	586.09 g/mol
Synonyms	D-Valyl-L-leucyl-L-arginine 4-nitroanilide hydrochloride; D-Val-Leu-Arg-pNA • HCl; H-D-Val-Leu-Arg-pNA hydrochloride; Chromogenic Substrate for Thrombin; Thrombin Substrate I; Tos-Gly-Pro-Arg-pNA analog; S-2238 Analog; Benzoyl-Phe-Val-Arg-pNA derivative; Protease Substrate 162303-66-4
EINECS	Contact for details

Quality Control

Our D-Val-Leu-Arg p-Nitroanilide Hydrochloride Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and stringent controls for residual solvents and moisture content. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Absorbance (λ max)	310 - 320 nm
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.