Description

Dabcyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-Edans is a specialized fluorogenic peptide substrate designed for the continuous, highly sensitive detection of caspase-3 and related caspase activity. This compound is critical for researchers and developers in life sciences, enabling precise measurement of apoptosis (programmed cell death) in biochemical assays and high-throughput screening. It is an essential tool for pharmaceutical R&D, oncology research, and cell biology studies where understanding and quantifying caspase activation is paramount.

Application

• Caspase-3 Activity Assays: Primary substrate for fluorometric and spectrophotometric determination of caspase-3 enzyme activity in vitro.
• Apoptosis Research: Used in cell-based and cell-free systems to study and quantify the induction of apoptosis in cancer research and toxicology.
• High-Throughput Screening (HTS): Enables the screening of large compound libraries for caspase-3 inhibitors or activators in drug discovery pipelines.
• Biochemical Reagent Manufacturing: A key raw material for producing commercial assay kits for apoptosis detection sold to research laboratories globally.
• Enzyme Kinetics Studies: Facilitates the study of enzyme kinetics, specificity, and inhibition profiles of caspase-3 and related proteases.

Basic Information

Product Name	Dabcyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-Edans
CAS No.	161877-70-9
Molecular Formula	C₆₀H₈₁N₁₃O₂₀
Molecular Weight	1280.4 g/mol
Synonyms	Caspase-3 Substrate IV; Ac-DEVD-pNA; Ac-Asp-Glu-Val-Asp-p-nitroanilide; Caspase 3 Fluorogenic Substrate; Ac-DEVD-AFC; (7-Methoxycoumarin-4-yl)acetyl-Asp-Glu-Val-Asp-Ala-Pro-Lys(DNP); Dabcyl-KTDEVDAPC-Edans; Fluorogenic Caspase-3 Substrate
EINECS	Contact for details

Quality Control

Every batch of Dabcyl-Tyr-Val-Ala-Asp-Ala-Pro-Val-Edans is manufactured and tested under strict quality management systems to ensure the highest standards of purity and performance for critical research applications. Our products undergo rigorous quality testing, including HPLC for purity and identity confirmation, to ensure compliance with industry standards. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.