Description

Pefachrome(R) Tpa* CAS NO 161572-29-8 is a high-purity chromogenic peptide substrate specifically designed for the selective detection and measurement of thrombin activity in biochemical assays. This compound is critical for researchers and quality control laboratories requiring precise, reliable, and reproducible results in coagulation studies and diagnostic development. It is an essential reagent for pharmaceutical R&D, clinical diagnostics, and biotechnology applications focused on hemostasis and thrombosis.

Application

Pefachrome(R) Tpa* is a specialized tool primarily used in enzymatic activity assays. Its key applications include:

• Thrombin Activity Assays: Quantitative measurement of thrombin concentration and activity in research samples.
• Diagnostic Kit Manufacturing: A key component in the production of in-vitro diagnostic (IVD) kits for coagulation disorders.
• Pharmaceutical Research & Development: Screening and evaluation of novel anticoagulant and procoagulant drug candidates.
• Quality Control in Biologics: Monitoring residual thrombin-like activity in downstream bioprocessing of therapeutic proteins.
• Clinical Laboratory Testing: Used in specialized clinical labs for detailed coagulation factor analysis.
• Enzyme Kinetic Studies: Determination of kinetic parameters (Km, Vmax) for thrombin and related serine proteases.

Basic Information

Product Name	Pefachrome(R) Tpa*
CAS No.	161572-29-8
Molecular Formula	C47H62N12O12
Molecular Weight	963.07 g/mol
Synonyms	Pefachrome tPA; Chromogenic Substrate tPA; Tosyl-Gly-Pro-Arg-p-nitroanilide acetate salt; Thrombin Chromogenic Substrate; H-D-CHT-Gly-Arg-pNA • AcOH; Pefachrome® TH; S-2238 Analog; Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride (related compound)
EINECS	Contact for details

Quality Control

Every batch of Pefachrome(R) Tpa* is manufactured and tested under strict quality management systems. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic methods, to ensure compliance with exacting research and diagnostic standards. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0 %
Solubility	Clear, colorless solution in water or buffer
Functional Activity	Conforms to expected δA/min/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.